新生犊牛皱胃平滑肌细胞的原代培养——组织块培养法和胶原酶消化法的比较研究  

Primary culture of abomasum smooth muscle cells in newborn calf Comparison research on methods of tissue culture and collagenase digestion

作　　者：许远靖[1] 刘芳宁[1] 贾艳[2] 田武林[1] 李彬[1] 魏腾[1] 张乃生[1] 靳朝[1] 刘国文[1] 谢光洪[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]嘉兴职业技术学院,浙江嘉兴314036

出　　处：《中国兽医学报》2012年第12期1872-1875,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31101865)

摘　　要：通过组织块培养法和胶原酶消化法分离培养犊奶牛皱胃平滑肌细胞,采用免疫细胞化学方法和免疫荧光化学方法对其进行鉴定,并将鉴定结果、细胞生长曲线进行比较。结果,两种方法培养出的细胞生长曲线基本相同,细胞纯度免疫细胞化学鉴定结果为:组织块培养法为95%以上,胶原酶消化法为90%;免疫荧光鉴定结果为:组织块培养法为96%,酶消化法为89%。组织块培养法操作简单、经济,但耗时较长;胶原酶消化法快速、获得细胞较多,但价格昂贵,故在实际操作中可将两种方法联合应用。Abomasum smooth muscle cells of newborn calf were isolated and cultured with tissue culture and collagenase digestion,and were indentified through immunocytochemistry and immunofluorescence technique, and then the results and the cell growth curves were compared. The cell growth curves based on the two ways were almost the same;the cell purity was more than 95 % through tissue culture,and 90 % through the collagenase digestion with the immunocytochemistry identification, which is almost the same to immunofluorescence results： 96% through tissue culture and 89 % through collagenase digestion respectively. Tissue culture method is easy to operate and economical,but takes a long time;collagenase digestion method is rapid, easy to obtain more cells, but very expensive, so we would like to use the tissue culture-in combination with collagenase digestion in practice.

关 键 词：奶牛 皱胃平滑肌细胞 培养 

分 类 号：S823[农业科学—畜牧学]