柱前衍生化HPLC法测定黄河滩枣多糖的单糖组成  

Determination of monosaccharide components of Huanghe jujube polysaccharides by pre-column derivatization HPLC method

作　　者：郝蕾蕾[1] 张典瑞[1] 赵忠熙[1] 刘燕[1] 

机构地区：[1]山东大学药学院药物制剂与释药系统研究中心,山东济南250012

出　　处：《中国生化药物杂志》2012年第6期740-743,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：国家重点基础研究发展计划(项目编号:2012CB126315)

摘　　要：目的建立PMP(1-苯基-3-甲基-5-吡唑啉酮)柱前衍生化HPLC法测定黄河滩枣多糖的单糖组成。方法采用水提醇沉法提取黄河滩枣多糖,多糖水解后进行PMP衍生化,HPLC法测定黄河滩枣多糖的单糖组成及摩尔比,并对等度和梯度洗脱两种模式的测定结果进行分析比较。结果黄河滩枣多糖由D-甘露糖、L-鼠李糖、D-半乳糖醛酸、D-葡萄糖、D-半乳糖、L-阿拉伯糖等六种单糖组成,它们的摩尔比约为0.54∶0.50∶0.27∶2.03∶5.40∶1.00,等度和梯度洗脱测定结果基本一致。结论本实验改进的等度洗脱方式更加简便易行,快速准确,适用于黄河滩枣多糖的单糖组成分析。Purpose To establish PMP pre-column derivatization HPLC method for determination of monosaccharide components of Huanghe jujube polysaccharides. Methods The polysaccharides of Huang- he jujube were extracted with water, precipitated by ethanol and then the monosaccharides were derivated with PMP after hydrolyzed. HPLC method was used to study the monosaccharide components and molar ra- tio of Huanghe jujube polysaccharides,and the two patterns of isocratic elution and gradient elution were compared and analyzed. Results Huanghe jujube polysaccharides were composed of D-mannose,L-rham- nose, D-galacturonic acid, D-glucose, D-galactose, and L-arabinose and their molar ratio was about 0.54： 0.50：0.27：2.03：5.40：1.00,with almost the same results of isocratic elution and gradient elution. Con- clusion The l^ocratic elution method used in this experiment is more simple,quick and accurate and can be used to analyze the monosaccharide components of Huanghe jujube polysaccharides.

关 键 词：黄河滩枣多糖 单糖组成 PMP柱前衍生 高效液相色谱 

分 类 号：R284[医药卫生—中药学]