重组人骨形成蛋白2/骨髓间充质干细胞复合磷酸三钙应用于脊柱融合的实验研究  

Experimental Research on Spinal Fusion with Recombinant Human Bone Morphogenetic Protein-2 and Bone Marrow Tromal Cell Composited Tricalcium Phosphate(TCP)

作　　者：杨小荣 吴良绍 方煌[2] 

机构地区：[1]湖北省恩施自治州中心医院骨科,恩施445000 [2]华中科技大学同济医学院附属同济医院骨科,武汉430030

出　　处：《生物医学工程学杂志》2012年第6期1156-1159,1167,共5页Journal of Biomedical Engineering

摘　　要：观察重组人骨形成蛋白2(rhBMP-2)与骨髓间充质干细胞(BMSCs)复合磷酸三钙(TCP)应用于大鼠腰椎横突间融合的愈合情况。体外培养大鼠BMSCs,20只SD大鼠均行单节段双侧L4,5横突间植骨融合术,依植入物的不同分为实验组与对照组,每组10只。实验组植入复合rhBMP-2和BMSCs的TCP,对照组只植入TCP。术后4周处死动物,取出腰椎,通过大体观察、手法检测、影像学、组织学等方法分析融合状况。结果表明应用rhBMP-2和BMSCs的TCP组新骨形成良好,脊柱融合效果好,生物力学强度高,融合率显著高于对照组(P<0.05)。结果提示:应用rhBMP-2和BMSCs有利于复合材料成骨和脊柱融合。This paper is aimed to assess the efficacy ot and bone marrow stromal cell （BMSCs） eomposited tricalcium phosphate （TCP） in a rat model of posterolateral lum bar intertransverse process fusion. Rat BMSCs were cultured in vitro. Twenty SD rats underwent singlelevel bilat eral intertransverse process spine arthrodesis at L4 and LS. These rats were assigned to two groups according to the graft materials. They received： 10 of the total were treated with the BMSCs with rhBMP2 and tricaleium phosphate （TCP） as the experimental group,and the other 10 with TCP treatment alone as the control group. All the animals were killed at 4 weeks after surgery and the spine fusion results were assessed by gross inspection, manual palpation, radiography and histology. The fusion rate, the tensile strength and stiffness of the solidly fused levels in the experi mental group were statistically higher than that of the controlled group（P〈0.05）. These results showed that the spinal fusion could be improved mechanically when rhBMP2 and BMSCs were added into the TCP.

关 键 词：重组人骨形成蛋白2 骨髓间充质干细胞 磷酸三钙 脊柱融合 

分 类 号：R318.08[医药卫生—生物医学工程]