流体剪切应力对成骨细胞信号调控网络影响的体外研究进展  

A Review on Progress of In Vitro Research of Fluid Shear Stress Influence on Signaling Networks of Osteoblasts

作　　者：朱婷[1] 董亚兵[1] 孙惠强[1,2] 

机构地区：[1]山东大学口腔医学院,济南250012 [2]山东省口腔生物医学重点实验室,济南250012

出　　处：《生物医学工程学杂志》2012年第6期1207-1211,共5页Journal of Biomedical Engineering

基　　金：山东省自然科学基金资助项目(ZR2010HM035);山东省卫生厅科技发展计划项目资助(2011WSB19002)

摘　　要：机械应力在骨的生长、重建过程中起着十分重要的作用。应力作用于骨组织对骨细胞、成骨细胞等应力感受细胞产生牵张应力和流体剪切应力(FSS),进而影响细胞内相关基因的表达,其中FSS占主导作用。FSS引起体外培养的成骨细胞分子活动的具体机制尚不明确,细胞内的Ca2+通路、环氧化酶—前列腺素E2通路、蛋白激酶A/蛋白激酶C(PKA/PKC)通路、Smad蛋白通路等可能在FSS介导的成骨细胞分化过程中起重要作用。本文通过对这些信号通路在FSS影响体外培养成骨细胞分化中的作用进行综述,了解FSS影响成骨细胞分子活动的应力传导以及信号转导的研究现状,为进一步研究其具体机制提供参考。Mechanical stress plays an important role in bone growth and bone remodeling. It causes stretch stress and fluid shear stress （FSS）, which can be sensed by mechanosensory cells, e.g. osteocytes and osteoblasts, and further induce changes of gene expression in those cells. The FSS is thought to be the main cause in this process. However, up to now, it is still not clear what signals are triggered in the mechanosensory cells cultured in vitro and how the FSS exactly affects the expression of specific proteins. Evidences have shown that Ca2＋ signaling pathway, Cyclooxygenase-prostaglandin E2 pathway, protein kinase A/protein kinase C （PKA/PKC） pathway, and drosophila mothers against decapentaplegic （Smad） protein pathway may be the key players in osteoblast differentiation by FSS. The precise mechanism involved in mechanotransduction and signal transduction remains to be elucidated. The present re- view gives a brief summary on the effects of these signaling pathways on the differentiation of osteoblasts cultured in vitro under FSS, to get the message of the present situation of the research on the stress transmission and signal transduction influencing osteoblast molecular activity, and to provide reference for further study on its specific mechanism.

关 键 词：应力感受细胞 流体剪切应力 成骨细胞 力学信号转导 

分 类 号：R318.01[医药卫生—生物医学工程]