rBPI_(23)基因克隆与鉴定  

Cloning and identification of rBPI_( 23)gene(BPI_(600 bp))

作　　者：柯岩[1] 安云庆[1] 杨贵贞[2] 

机构地区：[1]首都医科大学微生物学和免疫学教研室,北京100054 [2]白求恩医科大学免疫学教研室,长春130021

出　　处：《中国免疫学杂志》2000年第6期312-314,317,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金资助项目!(项目号:39570666)

摘　　要：目的 :rBPI2 3基因 (BPI60 0bp)克隆与鉴定。方法 :采用RT PCR技术 ,从HL 6 0细胞mRNA中扩增编码BPIN端 199个氨基酸 (rBPI2 3)的基因片段 (BPI60 0bp) ,经酶切后通过连接反应构建重组克隆载体 ,测序鉴定。结果 :RT PCR获得预期的扩增产物—BPI60 0bp基因片段。成功构建PUC18 BPI180 、PUC18 BPI4 2 0 重组克隆载体 ,酶切、酶谱分析与预期结果相符。DNA测序结果与文献报道一致。结论 :PUC18 BPI180 、PUC18 BPI4 2 0 重组克隆载体构建成功 ;BPI60Objective:To clone and identify BPI 600 bp gene which encode rBPI 23 .Methods:The gene which encode rBPI 23 were amplified by RTPCR from mRNA that were extracted from HL60.Recombinant cloning vector was constructed sequenced after the enzyme digestion.Results:①Prospective amplified productsBPI 600 bp gene was obtained by RTPCR method.②PUC18BPI 180 、PUC18BPI 420 cloning vector were successfully constructed,and the enzyme digestion analysis are identical with prospective results.③Sequences are identical with those of the report.Conclusion:PUC18BPI 180 、PUC18BPI 420 cloning vector were successfully constructed,and BPI 600 bp gene sequences are identical with those of the report.

关 键 词：PUC18克隆载体 RT-PCR rBPI23基因 

分 类 号：Q785[生物学—分子生物学]