咳嗽变异性哮喘患儿诱导痰神经生长因子和白细胞介素-4水平检测  

Levels of nerve growth factor and interleukin-4 in the induced sputum of children with cough variant asthma

作　　者：李红子[1] 金正勇[1] 元熙哲[1] 金春姬[1] 

机构地区：[1]延边大学附属医院儿科,吉林延边133000

出　　处：《中国当代儿科杂志》2012年第12期924-927,共4页Chinese Journal of Contemporary Pediatrics

摘　　要：目的检测咳嗽变异性哮喘(CVA)患儿诱导痰中神经生长因子(NGF)和白细胞介素-4(IL-4)的表达,探讨NGF和IL-4在小儿CVA中的作用。方法 34例CVA患儿纳入研究,20例健康儿童作为正常对照。对受试者进行痰诱导,苏木精-伊红染色计数诱导痰中嗜酸性粒细胞的百分比。酶联免疫吸附双抗体夹心法检测诱导痰液上清中NGF和IL-4的表达。荧光定量PCR检测诱导痰液沉淀细胞中NGF和IL-4 mRNA的表达。结果 CVA组患儿诱导痰中嗜酸性粒细胞百分数为(13.4±3.6)%,显著高于对照组的(2.6±1.7)%(P<0.01)。CVA组患儿诱导痰上清中NGF和IL-4的表达水平及诱导痰沉淀细胞中NGF和IL-4 mRNA的表达水平均显著高于对照组,差异有统计学意义(均P<0.05)。诱导痰上清中NGF和IL-4的表达水平及诱导痰中NGF和IL-4 mRNA的表达水平与嗜酸性粒细胞计数均呈正相关(均P<0.05)。CVA患儿治疗后诱导痰上清中NGF和IL-4的表达水平及诱导痰中NGF和IL-4 mRNA的表达水平均出现了显著的减低(均P<0.05)。结论嗜酸性粒细胞的浸润和炎性介质NGF、IL-4的升高参与了小儿CVA的发生发展过程,在小儿CVA的诊断和治疗中具有一定的价值。Objective To examine the levels of nerve growth factor（NGF） and interleukin-4（IL-4） in the induced sputum of children with cough variant asthma（CVA）,with the aim of studying the roles of NGF and IL-4 in childhood CVA.Methods Thirty-four children with CVA were enrolled in this study.Twenty healthy children were used as a normal control group.The induced sputum was separated into supernatant and cells.Hematoxylin and eosin staining was used to count differential cells.The expression of NGF and IL-4 in supernatant was measured using ELISA.The mRNA expression of NGF and IL-4 in cells was determined by Real-time PCR analysis.Results The percentage of eosinophils in the CVA group was significantly higher than in the control group [（13.4±3.6）% vs（2.6±1.7）%;P〈0.01].The expression of NGF and IL-4 protein and mRNA in induced sputum was significantly higher in the CVA group than in the control group（P〈0.05）.The expression of NGF and IL-4 protein and mRNA was positively correlated with the percentage of eosinophils（P〈0.01）.The expression of NGF and IL-4 protein and mRNA in induced sputum was significantly reduced in the CVA group after treatment（P〈0.05）.Conclusions Eosinophils infiltration and increased expression of NGF and IL-4 play key roles in the development of childhood CVA,suggesting that they may be useful in the diagnosis and treatment of childhood CVA.

关 键 词：咳嗽变异性哮喘 神经生长因子 白细胞介素-4 儿童 

分 类 号：R725.6[医药卫生—儿科]