左归丸对去卵巢大鼠股骨中Ⅰ型胶原mRNA表达的影响  

Expressions of collagen ⅠmRNA of femoral organization by Zuogui Pill in ovariectomy-induced osteoporosis rats

作　　者：王艳杰[1] 任艳玲[1] 宋囡[1] 李亚玲[1] 吕海波[1] 赵金茹[1] 

机构地区：[1]辽宁中医药大学基础医学院,辽宁沈阳110032

出　　处：《中国老年学杂志》2012年第23期5170-5172,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金项目(No.30873226);教育部高等学校博士学科点专项科研基金(No.20102133110001);辽宁省高等学校优秀人才支持计划(No.LR201025);辽宁省科技厅自然基金(No.201102148)

摘　　要：目的观察左归丸对去卵巢骨质疏松(OP)模型大鼠股骨中Ⅰ型胶原mRNA表达的影响,探讨左归丸对OP的防治作用机制。方法SPF级4.5月龄SD雌性大鼠,分为三组:正常组39只;假手术组39只;200只采取双侧背部卵巢切除术进行绝经后OP造模。造模21 d后,将去卵巢大鼠随机分为5组:模型组、尼尔雌醇组、左归丸高剂量组、左归丸中剂量组、左归丸低剂量组,每组40只。然后开始灌胃给药,左归丸高、中、低剂量组分别给予ig6.4,3.2,1.6 g/kg剂量的左归丸混悬液灌胃;尼尔雌醇组灌服尼尔雌醇混悬液灌胃;正常组、假手术组和模型组均灌服蒸馏水。于连续给药60、120、180 d后分3批取材,取大鼠左后肢股骨近端1/3部分,采用RT-PCR方法检测骨组织中Ⅰ型胶原mRNA的表达水平。结果去卵巢后,与正常组比较,模型组大鼠股骨中Ⅰ型胶原mRNA水平明显降低(P<0.01);和模型组比较,给药60d、120、180 d的左归丸各剂量组,股骨中Ⅰ型胶原mRNA水平显著升高(P<0.01或P<0.05)。结论骨组织中Ⅰ型胶原的mRNA表达水平的下调可能是绝经后骨质疏松(PMOP)发生的重要机制之一,左归丸能够上调骨组织中Ⅰ型胶原的mRNA表达,从而有效防治OP。Objective To observe the expression of collagen ⅠmRNA of femoral organization by Zuogui Pill in ovariectomy-induced osteoporosis rats. Methods 280 male SPF SD rats were divided into normal and sham groups （n = 39） and the other 195 rats were divided into ovariectomy-induced osteoporosis model group. 21 days later, osteoporosis model rats were randomly divided into 5 groups： the model, nilestriol, high, middle and low dose of Zuogui pill groups （n = 39）. Then rats were given i.g. with Zuogui Pill at dosage of 6.4, 3.2, 1.6 g · kg^-1 · d^-1 and nilestriol respectively. Rats in normal, sham and the model groups were given distilled water. After treated with medicine 60, 120, 180 days, the expression of collagen Ⅰ in the femoral organization was examined by RT-PCR. Results Compared with the normal group, the femoral collagen Ⅰ mRNA level obviously was reduced in model group （P 〈 0.01 ）. Compared with the model group, collagen Ⅰ mRNA level was significantly increased in Zuogui Pill groups （ P 〈 0.01 or P 〈 0.05 ）. Conclusions Expression of collagen Ⅰ mRNA of bone tissue is down-regulated in postmenopausal osteoporosis, Zuigui pill can be up-regulated mRNA expression of collagen Ⅰ in bone tissue.

关 键 词：左归丸 骨质疏松症 Ⅰ型胶原 

分 类 号：R289.5[医药卫生—方剂学]