虾青素对骨关节炎软骨细胞氧化损伤及炎性的影响  

Study of Astaxanthin on anti-oxidative damage and anti-inflammatory of osteoarthritis cartilage cells

作　　者：裴凌鹏[1] 白岩[1] 惠伯棣[2] 

机构地区：[1]中央民族大学中国少数民族传统医学研究院国家民委-教育部重点实验室,北京100081 [2]北京联合大学应用文理学院

出　　处：《中国老年学杂志》2012年第23期5182-5184,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助(No.30902011);教育部"中央高校基本科研业务费专项资金"资助(No.0910KYCZ01);教育部"长江学者和创新团队发展计划"资助(IRT0871)

摘　　要：目的研究虾青素对骨关节炎(OA)软骨细胞氧化损伤及炎性的逆转作用。方法选择15只5月龄新西兰兔,采用内侧副韧带、前后交叉韧带切断并切除内侧半月板的OA动物模型制作方法,体外分离培养软骨细胞。以假手术组细胞为正常对照组,造模组细胞为OA软骨细胞,分别加入10、20、30μg/ml虾青素(低、中、高剂量);从第9周开始,每周处死1组动物,分离培养软骨细胞。连续8 w,DCFH-DA法检测细胞内活性氧(ROS)水平,Griess法测定培养上清中氧化氮(NO)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)含量,RT-PCR法检测各组细胞相关炎性因子(IL)-1β、诱导型一氧化氮合酶(iNOS)、转录调节因子p53基因mRNA的表达。结果 DCFH-DA法检测细胞内ROS水平,对照组与模型组差异显著(P<0.01);虾青素低、中、高剂量组与模型组差异显著(P<0.01)。模型组中NaNO2、SOD、GSH-Px与对照组差异显著(P<0.01);NaNO2、SOD、GSH-Px水平虾青素低、中、高剂量组与模型组差异显著(P<0.01,P<0.05)。模型组中IL-1β、iNOS、p53基因mRNA的表达量与对照组及各剂量虾青素组差异显著(P<0.01)。结论虾青素对OA软骨细胞的氧化损伤及炎性有逆转作用,具有研发成为OA治疗药物的潜能。Objective Objective ：To study the reverse effect of the oxidative damage and inflammatory on cartilage cells by Astaxanthin. Methods Fifteen New Zealand white rabbits of 5 month old were selected. Osteoarthritis cartilage cells in model groups were added Astaxanthin 10,20,30 μg/ml respectively. A group of animals were sacrificed every week from the ninth weeks and the cartel age ceils were isolated and cultured. For 8 w, the level of reactive oxygen species （ROS） in chondrocyte was detected by DCFH-DA,the content of NO and the activity of SOD and GSH-Px in cell culture supernatant were detected by Griess method. The related expression contents of IL-1 β/GAPDH, iNOS/GAPDH and p53/GAPDH were detected by RT-PCR. Results DCFH-DA detection of intracellular reactive oxygen species in control group was less than that in model group and that in Astaxanthin groups was less than that in model group（P 〈0. 01 ）. The contents of NaNO2, SOD and GSH-Px in osteoarthritis model group had significant differences with those of control group （P 〈 0.01 ） ;and those in Astaxanthin groups had significant differences with those of control group（ P 〈 0. 01 ）. The related expression contents of IL-1 β/GAPDH, iNOS/ GAPDH ,p53/GAPDH in Astaxanthin groups were significant with model group. Conclusions Astaxanthin has anti-oxidative damage and anti-inflammatory effect of osteoarthritis cartilage ceils within a certain dose range. It might be the main mechanism for astaxanthin to treat osteoarthritis.

关 键 词：虾青素 骨关节炎 软骨细胞 氧化损伤 炎性因子 

分 类 号：R68[医药卫生—骨科学]