含弓形虫ROP1基因真核表达重组质粒DNA免疫小鼠的研究 IV.免疫鼠血清细胞因子IFN-γ、IL-2及NO的测定  

STUDIES ON IMMUNO-RESPONSE OF DNA VACCINATION WITH RECOMBINANT PLASMID pcDNA3 CONTAINING ROP1 GENE FROM TOXOPLASMA GONDII IN BALB/C MICE IV.The Detecting of IFN-γ,IL-2,and NO in the Serum from the Immunized Mice

作　　者：郭虹[1] 陈观今[1] 郑焕钦[1] 

机构地区：[1]中山医科大学寄生虫学研究所,广州510089

出　　处：《中国人兽共患病杂志》2000年第4期18-20,共3页Chinese Journal of Zoonoses

基　　金：国家自然科学基金资助项目!编号3860451;中山医科大学"211工程"重点学科建设课题!编号201053-1104

摘　　要：目的　用 pc- ROP1重组质粒免疫小鼠 ,观察其对细胞因子 IFN-γ、IL - 2及 NO的影响。方法　碱裂解法大量制备 pc- ROP1质粒 DNA,经肌肉注射免疫 BAL B/ c小鼠 ,每只鼠注射 10 0μg,两周后同量加强免疫一次 ,以 pc DNA 3空质粒及生理盐水组为对照。分别于免疫后第 30天、5 0天、70天共三次用双夹心 EL ISA测定血清细胞因子 IFN-γ、IL - 2含量 ;酶法测定NO活性。结果　三次检测免疫组 IFN-γ、IL - 2及 NO含量均显著高于对照组 ,随着免疫时间的延长有增高趋势。结论　 pc-ROP1重组质粒 DNA免疫小鼠 ,可刺激细胞因子 IFN-γ、IL - 2产生 。Aim DNA vaccinating BALB/c mice with the constructed recombinant plasmid, pcDNA3, containing ROP1 gene from Toxoplasma gondii to observe the effect on the production of the cytokines, IFN-γ、IL-2,and NO in the immunized mice.Methods Large-scale preparation of plasmid DNA by alkaline lysis,the DNA were injected through muscles of left leg in each mouse at the dosage of 100μg. A booster vaccine was given at the same dosage after two weeks. Control groups were injected with pcDNA3 blank plasmid and normal saline respectively. After 30,50 and 70 days of the booster injection, following tests were carried out 3 times separately:the serum IFN-γand IL-2 were detected by sandwich ELISA;the NO was detected by enzyme assay.Results The 3 times detected results ofIFN-γ、IL-2 and NO were significant higher in the vaccinated group than that of in control groups and the contents were increased with the vaccinated time prolonged. Conclusion The IFN-γ、IL-2 and NO were produced by vaccinating BALB/c mice with the recombinant plasmid, pc-ROP1.

关 键 词：弓形虫病 棒状体蛋白 DNA免疫 RO01基因 质粒 

分 类 号：R531.8[医药卫生—内科学]