日本血吸虫肠道蛋白酶对小鼠和东方田鼠血红蛋白降解作用的比较研究  

COMPARATIVE STUDY ON DEGRADATION OF HEMOGLOBINS IN MOUSE AND MICROTUS FORTIS BY SCHISTOSOMA JAPONICUM DIGESTING PROTEINASES

作　　者：王庆林[1] 易新元[1] 罗新松[2] 何永康[2] 彭兴华[3] 

机构地区：[1]湖南医科大学寄生虫学教研室,长沙410078 [2]湖南省寄生虫病防治研究所 [3]湖南医科大学分子生物学中心

出　　处：《中国人兽共患病杂志》2000年第4期35-36,共2页Chinese Journal of Zoonoses

基　　金：国家九五科技攻关项目!(专题编号:96-A23-06-04)

摘　　要：目的　从日本血吸虫 (Schistosoma japonicum,Sj)对宿主血红蛋白 (hem oglobin,Hb)的消化作用来探讨 Sj在东方田鼠 (Microtus fortis,Mf)体内不能正常发育的原因。方法　用 Sj肠道蛋白酶对小鼠和 Mf Hb在体外进行消化 ,用 SDS-PAGE显示不同时点 Hb的降解程度。结果　 Sj肠道蛋白酶能有效降解 Mf Hb,且与其对小鼠 Hb的降解无明显差别。结论　Sj在 Mf体内不能正常发育的原因似与 Sj对 MfAIM To explore mechanisms of Schistosoma japonicum(Sj) underdevelopment and eventual death in Microtus fortis (Mf) in terms of host hemoglobin(Hb)digestion. METHOD The vomitus of Sj was induced and collected in distilled water. Mouse and Mf Hb were digested in vitro with Sj vomitus. The results of Hb degradation were examined by SDS-PAGE. RESULTS Sj digestive proteinases could degrade Mf Hb effectively. There was no obvious difference in degradation between Mf Hb and mouse Hb. CONCLUSION Sj does not develop normally in Mf may probably not be related to the digestion of Mf Hb.

关 键 词：日本血吸虫病 肠道蛋白酶 东方田鼠 血红蛋白 

分 类 号：R532.21[医药卫生—内科学]