几种弓形虫ELISA试剂盒初步检测结果比较  

COMPARISON OF PRELIMINARY DETECTION RESULTS OF SEVERAL TOXOPLASMA GONDII ELISA TEST KITS

作　　者：牛安欧[1] 冯友仁[1] 刘文琦 

机构地区：[1]同济医科大学寄生虫学教研室,武汉430030

出　　处：《中国人兽共患病杂志》2000年第4期56-58,共3页Chinese Journal of Zoonoses

摘　　要：本文选用 5种国内弓形虫 EL ISA试剂盒检测已知同样样品 ,并且用国外试剂盒核查由该 5种试剂盒提供的阳性样品 ,作试剂盒质量比较。结果　 Ig G- EL ISA试剂 A、B盒阳性检出率 (敏感性 )均为 6 0 % ,A、B盒之间的符合率仅为5 0 % ,其特异性分别为 35 %和 90 % ;C、D、E阳性检出率 (敏感性 )分别为 30 %、6 0 %、70 % ,特异性分别为 75 %、5 4%、87%。核查 A、B、D、E盒提供的样品结果 :Ig G符合率较好 ,均在 90 %以上。 Ig M符合率均为 6 0 %。鉴于目前国内 EL ISA试剂盒的现状 ,本文分析后建议 ,除改进质量外 ,宜选择多种试剂盒联合应用 ,避免漏检和误诊。Aim Five types of civil Toxoplasma gondii ELISA test kit were used to the detected specimen which proved to be the same, foreign-made test kit was used to examine the positive specimen given by these five types of test kit, as to make comparison about quality of test kit. Results IgG-ELISA A,B kits positive showing rates (sensitive)are all 60%,the corresponding rate between A&B is merely 50%,the specifics are 35%&90%.C,D,E kits' positive showing rates(sensitive)are 30%,60%,70% the specifice are 75%,54%,87%.The result about examining the probes given by A,B,D,E kit:IgG corresponding rates are better, which are all more than 90%;IgM corresponding rates are all more than 60%.It turn out that, according to the civil conditions of ELISA test kit, we suggest that besides the quality being improved in, all kinds of test kit should be used combinatively, so as to avoid neglect and mistake on examination, and we should be cautious in examining.

关 键 词：弓形虫病 ELISA 试剂盒 

分 类 号：R531.804[医药卫生—内科学] R446.61[医药卫生—临床医学]