预防性使用刺五加皂甙对海马伞切割后大鼠学习记忆功能的改善作用  

Effects of pretreatment with acanthopanax senticousus saponins on learning and memory ability following fimbrial transection in rats

作　　者：陈翔[1] 顾永健[1] 

机构地区：[1]南通大学附属医院神经内科,南通226001

出　　处：《上海交通大学学报（医学版）》2012年第12期1600-1604,共5页Journal of Shanghai Jiao tong University:Medical Science

摘　　要：目的观察预防性使用刺五加皂甙(ASS)对切割海马伞的大鼠学习记忆功能的影响及胆碱能神经元的变化。方法将27只SD大鼠随机分为ASS预防组、ASS治疗组和对照组,每组9只。在行右侧海马伞切割后,对照组每天腹腔注射生理盐水0.5 mL/100 g,ASS治疗组每天腹腔注射ASS 100 mg/kg(共6周);ASS预防组连续2周每天预先腹腔注射ASS 100 mg/kg后行右侧海马伞切割术,术后继续注射4周。切割海马伞术后第7、8周,分别进行避暗回避试验和跳台试验。术后第9周取大鼠切割海马伞侧隔区切片行还原型尼克酰胺腺嘌呤二核苷酸脱氢酶-黄递酶(NADPH-d)组织化学染色及胆碱乙酰转移酶(ChAT)免疫组织化学染色,分析一氧化氮合成酶(NOS)及ChAT阳性神经元数量、面积和周径。结果 ASS预防组大鼠在避暗回避试验中的探索次数和滞留时间,在跳台试验中的主动回避阳性率及总回避阳性率均优于ASS治疗组和对照组,差异有统计学意义(P<0.05);ASS治疗组大鼠避暗回避试验中的滞留时间明显长于对照组(P<0.05)。ASS预防组大鼠切割侧隔区的NOS和ChAT阳性神经元数量、面积及周径均显著大于治疗组和对照组(P<0.05);ASS治疗组大鼠切割侧隔区的NOS和ChAT阳性神经元面积和周径均明显大于对照组(P<0.05)。结论预防性使用ASS对切割海马伞大鼠的认知障碍有一定的改善作用,可能是通过保护基底前脑隔区胆碱能神经元而实现的。Objective To investigate the effects of pretreatment with acanthopanax senticousus saponins （ASS） on learning and memory ability and cholinergic neurons following fimhrial transection in rats. Methods Twenty-seven SD rats were randomly divided into ASS pretreatment group, ASS treatment group and control group, with 9 rats in each group. After right-side hippocampal fimhrial transection, 0.5 mL/100 g normal saline and 100 mg/kg ASS were intraperitoneally injected per day in control group and ASS treatment group respectively for 6 weeks. In ASS pretreatment group, rats were treated with intraperitoneal injection of 100 mg/kg ASS per day for 2 weeks before right-side hippocampal fimbrial transaction and for 4 weeks after operation. Seven weeks and eight weeks after fimbrial transection, dark avoidance test and passive and active avoidance test were conducted respectively in all groups. Nine weeks after fimbrial transection, septal area brain sections were obtained, immunohistochemieal staining of nicotinamide adenine dinncleotide phosphate-diaphorase （NADPH-d） and choline acetyhransferase （CHAT） was performed, and the numbers, areas and circumferences of nitric oxide synthetase （NOS） and ChAT positive neurons were analysed. Results The acquisition frequency and retention time in dark avoidance test and the positive rates of active and total avoidance reaction in passive and active avoidance test in ASS pretreatment group were superior to those in ASS treatment group and control group （P 〈 0.05）. The retention time in dark avoidance test in ASS treatment group was significantly longer than that in control group （ P 〈 0.05）. The numbers, areas and circumferences of NOS and ChAT positive neurons on the side of fimbrial transection in ASS pretreatment group were significantly greater than those in ASS treatment group and control group （P 〈 0.05）. The areas and circumferences of NOS and ChAT positive neurons on the side of fimbrial transection in ASS treatment group were significantly gr

关 键 词：刺五加皂甙 学习记忆 海马伞 一氧化氮合成酶 胆碱乙酰转移酶 大鼠 

分 类 号：R285.5[医药卫生—中药学]