三才刺透灸并用对肾结石大鼠肾脏及输尿管病理形态及生化指标的影响  

Influence of Electroacupuncture Combined with Intensive Moxibustion Intervention on Pathological Changes of Kidney and Ureter Tissues,Plasma and Uterine Ca^(2+),Creatinine,and Urea Nitrogen Levels in Calcium Oxalate Stone Rats

作　　者：王巍[1] 张洁[2] 

机构地区：[1]辽宁中医药大学针灸推拿学院,沈阳110032 [2]宁夏医科大学总医院康复医学科,银川750004

出　　处：《针刺研究》2012年第6期464-469,共6页Acupuncture Research

基　　金：辽宁省教育厅创新团队课题(2008T120)

摘　　要：目的:观察不同针灸法对肾草酸钙结石模型大鼠肾脏及输尿管病理形态及生化指标的影响,探讨取得最佳效应的特异针灸手法及其机制。方法:雄性Wistar大鼠60只随机分为空白组、模型组、常规针灸组、三才刺透灸Ⅰ组、三才刺透灸Ⅱ组,每组12只。采用1%乙二醇加1%氯化铵水作为诱石剂建立草酸钙结晶形成肾结石大鼠模型。常规针灸组常规针刺"膀胱俞""三阴交""志室",三才刺透灸组采用"天、人、地"三部刺入"肾俞"和灸热透达深层组织的三才刺透灸并用法,每日治疗1次,治疗7d。Von-kossa’s染色观察各组大鼠肾脏及输尿管组织的病理改变,分光光度计法测血浆钙离子(Ca2+)、尿中Ca2+水平,肌氨酸氧化酶法测血浆肌酐(Cr)、尿Cr水平,酶偶联速率法测血浆尿素氮(BUN)水平,光电比色法测尿酸(UA)水平。结果:与模型组比较,三才刺透灸并用法和常规针灸并用法均能减轻肾小球内充血,吸收炎性细胞,减轻炎性水肿,修复输尿管内坏死脱落的黏膜组织,降低血浆Ca2+、Cr、BUN水平及尿中Ca2+、Cr、UA的浓度(P<0.05,P<0.01),三才刺透灸并用法疗效较常规针灸组更显著(P<0.05,P<0.01)。结论:三才刺透灸并用法对大鼠肾草酸钙结石的影响优于常规针灸法,其作用机制可能为减轻肾脏及输尿管组织的病理改变,降低血、尿中Ca2+的含量,改善肾功能。Objective To observe the effect of electroacupuncture （EA） stimulation combined with moxibustion （MOX） on changes of the kidney and ureter tissue, plasma and uterine Ca^2＋ , creatinine （Or） and urea nitrogen （UN） concentrations in rats with renal calcium oxalate stone, so as to reveal its mechanism underlying relieving kidney stone. Methods A total of 60 Wistar rats were randomized into control group, model group, EA plus MOX of ＂Pangguangshu＂ （BL 28）, ＂Sanyinjiao＂ （SP 6） and ＂Zhishi＂ （BL 52） （routine EA-MOX group）, EA-intensive （INT） MOX of ＂Shenshu＂ （BL 23, EA-INT-MOX-BL 23 group） and EA-INT-MOX-Ashi group （12 rats/group）. Renal calcium oxalate stone model was established by feeding the animal with 1% eth- ylene alcohol and 1% ammonium chloride for 7 days. EA was applied to BL 28, SP 6, BL 52, BL 23, and Ashi-point for 15 min, respectively. For rats of the EA-INT-MOX-BL 23 group, EA intervention was applied to the superficial, medium and deep layers of BL 23 for 5 min, respectively, and INT MOX was given for 11 - 13 min. Plasma and uterine Ca^2＋ conctents were detected by ab- sorption spectrometry, plasma and urine Or contents determined by sarcosine oxidase method. Blood UN （BUN） level was deter- mined by enzyme coupling rate method and uric acid （UA） detected by photoelectric colorimetry method. Results Compared with the control group, inflammatory cell infiltration, congestion, space enlargement in the glomcrulus and renal interstitium, and unsmooth of the ureter wall with exfoliated necrosis tissue in the ureter were seen in the model group. These situations were rela- tively milder in the routine EA-MQX, EA-INT-MOX-BL 23 and EA-INT-MOX-Ashi groups. In comparison with the control group, plasma and uterine Ca^2＋ Cr, BUN, and UA levels were increased significantly in the model group（P〈0.01）. While compared to the model group, plasma and uterine Ca^2＋ Cr, BUN, and UA levels were down-regulated considerably （P〈0.05, P〈0.01 �

关 键 词：肾结石 针灸疗法 电针 艾灸 血浆 尿钙离子 肌酐 尿素氮 

分 类 号：R692.4[医药卫生—泌尿科学]