卵圆孔未闭动物模型的制备  

The preparation of patent foramen ovale model in dogs

作　　者：高力明[1] 宗书峰[1] 游晓华[1] 徐荣良[1] 秦永文[1] 

机构地区：[1]第二军医大学长海医院心内科,上海200433

出　　处：《介入放射学杂志》2012年第12期1011-1014,共4页Journal of Interventional Radiology

摘　　要：目的探讨采用经皮房间隔穿刺和球囊扩张法制备犬卵圆孔未闭(patent foramen ovale,PFO)动物模型的可行性。方法采用22只健康成年犬,经皮房间隔卵圆窝穿刺、球囊扩张术制备犬PFO模型,对其中2只犬的卵圆窝穿刺点进行病理学等检查。结果 22只犬均成功进行房间隔卵圆窝穿刺和球囊扩张术,右房造影可见对比剂由右房进入左房,成功建立了犬PFO模型。完成房间隔卵圆窝穿刺及球囊扩张平均用时(25.1±5.1)min,X线曝光时间平均(5.8±1.6)min。穿刺前右心房平均压为(1.9±0.8)mmHg,穿刺后测得左心房平均压为(2.5±0.7)mmHg。对2只犬的心脏大体观察见心包膜完整;房间隔卵圆窝缺损分别为5 mm×6 mm和6 mm×6 mm,缺损周边均见少量淤血,未见血栓附着,余心内膜均完整。结论经皮穿刺犬的房间隔卵圆窝、球囊扩张方法制备PFO模型可行。Objective To investigate the feasibility of establishing animal model of patent foramen ovale （PFO） in dogs by percutaneous atrial septal puncture and balloon dilation. Methods A total of 22 healthy adult dogs were selected for this study, The PFO models were established by percutaneous puncture of the atrial septa and balloon dilation of the fossa ovalis. Two dogs were randomly selected and sacrificed, and the tissues of fossa ovalis around the punctured site were removed for pathological examination. Results The percutaneous puncture of the atrial septa and balloon dilation of the fossa ovalis were successfully carried out in all the dogs. Angiography of the right atrium performed immediately after the procedure showed that the contrast medium flew from the right atrium into the left atrium in all dogs, indicating that PFO models were successfully established. The mean time for the performance of percutaneous puncture of the atrial septa and balloon dilation of the fossa ovalis was（25.1 ± 5.1） min. The mean X-ray exposure time was （5.8 ± 1.6） min. Before puncturing the atrial septa the mean pressure of the right atrium was （1.9 ± 0.8） mmHg, which was increased to （2.5 ± 0.7） mmHg after puncturing the atrial septa. Gross examination of the heart samples from the two sacrificed dogs showed that the cardiac pericardium was intact. The size of the fossa ovalis defect in the two dogs was 5 mm ×6 mm and 6 mm × 6 mm respectively. A little amount of ecchymosis was observed around the fossa ovalis defect, and no attached thrombus formation was observed. The rest part of the endocardium was well intact. Conclusion It is feasible to establish PFO model in dogs by percutaneous atrial septal puncture and balloon dilation.

关 键 词：卵圆孔未闭 房间隔穿刺 动物模型 实验研究 

分 类 号：R541.1[医药卫生—心血管疾病]