电磁辐射急性暴露对小鼠IL-12表达的影响及其机制  

Electromagnetic radiation enhances IL-12 expression in vivo and in vitro

作　　者：王源[1] 杨举[1] 张广斌[1] 余争平[1] 

机构地区：[1]第三军医大学军事预防医学院劳动卫生学教研室,电磁辐射医学防护教育部重点实验室,重庆400038

出　　处：《第三军医大学学报》2012年第24期2459-2464,共6页Journal of Third Military Medical University

基　　金：国家自然科学基金面上项目(30972432)~~

摘　　要：目的观察电磁辐射对昆明种小鼠白细胞介素-12(IL-12)表达的影响并探讨其机制。方法使用平均功率密度为90 mW/cm2900 MHz电磁辐射对昆明种小鼠及原代培养的小鼠腹腔巨噬细胞(Mφ)进行急性辐照。辐照后,ELISA检测IL-12表达量、RT-PCR检测IL-12p40亚基mRNA表达水平、Western blot检测Mφ细胞质IκB水平以及EMSA检测MφNF-κB转录活性的变化,观察吡咯烷二硫代氨基甲酸盐(pyrrolidinedithiocarbamate,PDTC)对电磁辐射影响IL-12表达的干预作用。结果急性辐照后第5天,小鼠脾脏组织IL-12p40 mRNA表达水平以及小鼠血清中IL-12p70含量达到峰值,增幅分别为9.32%、259.76%,与正常组相比差异有显著性(P<0.05),辐照后第11天恢复至正常水平。辐照后8 h,小鼠腹腔巨噬细胞(Mφ)细胞质中IκB-α含量降到最低,降幅为27.97%;辐照后12 h,小鼠腹腔巨噬细胞IL-12p40mRNA表达水平及培养上清中IL-12p70含量达到峰值,与正常对照组相比有显著性差异(P<0.01)。急性辐照后,各时相点均出现明显的NF-κB-探针结合带,辐照后8 h最为显著,正常对照组则检测不出明显的结合带。PDTC能够抑制电磁辐射急性暴露导致的IL-12高表达,抑制率为(83.43±7.58)%。结论 900 MHz电磁辐射急性暴露通过增加NF-κB活性,导致IL-12表达量增高。Objective To determine the expression of interleukin-12 （IL-12） in the cultured peritoneal macrophages （MФ） and in mice after electromagnetic radiation. Methods Kunming mice and primarily cultured MФwere radiated in 900 MHz electromagnetic field with an average powe r density of 90 mW/cm2 for 20 and 10 min respectively. RT-PCR was used to detect the expression of IL-12p40 in the spleen and cultured MФ at mRNA level. ELISA was employed to measure the levels of IL-12p70 in the serum and the MФ supernatant. Western blotting and EMSA was used to test IκB-α expression and transcriptional activity of NF-κB respectively in the cultured MФ. The intervention effect of pyrrolidinedithioearbamate （PDTC） , a NF-κB specific inhibitor, on the change of IL-12 expression after electromagnetic radiation was also observed. Results In 5 d after radiation, the expression level of IL-12p40 mRNA and the content of IL-12p70 in the serum reached a peak with a increase of 9.32% and 259.76% respectively, significantly higher than those in normal control （P 〈 0. 05）. The content of IL-12p70 and the expression of IL-12p40 mRNA in mice returned to normal level in 11 d after irradiation. The content of IκB-ct in cultured MФ was reduced to a minimum in 8 h after radiation with a drop of 27.97% , significant lower than that of normal group （P 〈0. 01 ）. In 12 h after acute radiation, the ex- pression of IL-12p40 mRNA in cultured MФ and the content of IL-12p70 in culture medium was increased reached a peak, significantly higher than those in the normal control group （ P 〈 0. 01 ）. After acute radiation,the NF-κB-probe binding activity was increased, and reached to the strongest in 8 h after radiation. PDTC resulted in a decrease in enhanced IL-12 expression induced by electromagnetic radiation, with an inhibitory rate of （83.43 ±7.58 ） %. Conclusion Acute exposure to 900 MHz electromagnetic radiation increases the expression of IL-12 by up-regulating NF-κB activity.

关 键 词：电磁辐射 IL-12 NF-ΚB IΚB-Α 

分 类 号：R146[医药卫生—公共卫生与预防医学] R329.11