7处移植公鱼种类的分子鉴定  

Molecular identification of transplanted Hypomesus species in seven places

作　　者：龚小玲[1] 吴颖[1] 崔忠凯[1] 鲍宝龙[1] 

机构地区：[1]上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室,上海201306

出　　处：《水产学报》2012年第12期1802-1808,共7页Journal of Fisheries of China

基　　金：上海市科技攻关项目(10dz1200804);上海市自然科学基金项目(09ZR1421700);上海教委创新项目(09YZ279);上海市重点学科(S30701)

摘　　要：公鱼属种类曾在中国被广泛移植,但被移植的种类却存在一定的争议,实验利用COⅠ基因序列片段对来自黑龙江、新疆和云南3地7处移植公鱼属种类进行了鉴定,并对它们之间的遗传关系进行了探讨。与GenBank和DNA Barcoding鉴定中心的条形码网站BOLDSystems进行比对,发现3地7处的移植公鱼均与西太公鱼序列相似度达到99%以上;移植公鱼及西太公鱼群体间和群体内的遗传距离均在0.1%～0.3%,与公鱼属其它种类的种间遗传距离则在10%以上;在NJ、Bayesian系统树上,移植公鱼、西太公鱼聚为同一单系,支持率超过98%;以上结果都证明这些移植公鱼均为西太公鱼。研究表明,7处移植公鱼都受到环境的选择压力,但群体间的遗传分化不显著(P=0.066 47>0.05)。在单倍型网络结构图中,它们之间遗传关系和移植路径不明晰,而多次和交叉移植可能是原因之一。The fishes of smelt genus Hypomesus lives in fresh water,brackish water and coastal seas,distributes in the Northern Hemisphere.Now,the Japanese smelt H.nipponensis and the pond smelt H.olidus are the common species and mainly distributed in the northeast region in China.Their bodies are small with silvery white.The life span of the fishes is short and theirs age of sexual maturity is above 1 year and they dies after spawning.They have strong adaptability and have been transplanted widely in China as the important commercial fishes.However,it is strange that there still exist disputation so far on which species of Hypomesus ever been transplanted widely in China.In this study,transplanted Hypomesus collected from seven places locating in Heilongjiang,Xinjiang and Yunnan province are identified using CO I gene,which is a popular DNA barcoding for species identification in the world.Comparative analysis showed that CO I gene sequence from all above collected somelts shared more than 99% similarity with that from H.nipponensis,which was published on the webs of GenBank and DNA barcoding Center.Genetic distance within each group and pairwise distance between transplanted smelt and H.nipponensis both ranged from 0.1% to 0.3%,and were rather smaller than those genetic distances between transplanted smelt and other species of Hypomesus（all more than 10%）.The topology of each final tree further revealed that all individuals of transplanted smelt and H.nipponensis formed a strong monophyletic group.Taken together,these results all showed that the transplanted smelt were Japanese smelt H.nipponensis.In addition,even the genetic differentiation in transplanted smelt populations was not significant（P = 0.066 47 0.05）,six populations of transplant Japanese smelt endured somewhat selection pressure of environment based on Tajima＇s D value.Median-joining haplotype network for Hypomesus 7 transplanted populations can not tell us too much about their genetic relation and the transplant routes.Cross-transplanted or/and m

关 键 词：移植公鱼 西太公鱼 物种鉴定 COⅠ基因 

分 类 号：Q958.8[生物学—动物学] S917.4[农业科学—水产科学]