脊尾白虾3个野生群体遗传多样性的微卫星分析  

Microsatellite analysis of genetic diversity in 3 wild populations of Exopalaemon carinicauda

作　　者：贾舒雯[1,2] 刘萍[2] 李健[2] 李吉涛[2] 高保全[2] 陈萍[2] 潘鲁青[1] 

机构地区：[1]中国海洋大学水产学院,山东青岛266003 [2]中国水产科学研究院黄海水产研究所,山东青岛266071

出　　处：《水产学报》2012年第12期1819-1825,共7页Journal of Fisheries of China

基　　金：国家"八六三"高技术研究发展计划(2012AA100809);山东省科技发展计划项目(2011GHY11526)

摘　　要：利用12对微卫星标记分析了莱州湾（LZ）、海州湾（HZ）、象山（XS）脊尾白虾野生群体的遗传多样性。12个位点在3个群体中均表现出高度的多态性。12个微卫星位点共得到115个等位基因,各个位点的等位基因数介于6~14,平均每个位点上的等位基因数为9.583 3个;各个位点的平均期望杂合度（He）、平均观测杂合度（Ho）、平均多态信息含量（PIC）分别为0.8278、0.517 5、0.705 1,表明3个脊尾白虾野生群体均有良好的多态性。经F-统计分析,各个群体间的遗传分化指数均值为0.093 0（0.The Exopalaemon carinicauda,subordinated to Decapoda,Palaemonidae,is an endemic shrimp species in China and is widely distributed in the coastal waters of China and west coast of the Korean Peninsula,especially in the Yellow Sea and Bohai Sea.So far,the E.carinicauda,has been studied on aquaculture,biology and ecology,but few reports were about population genetics.Fisheries management and conservation of this important marine species rely on a better understanding of its population genetics.So in this study,genetic diversity of 3 wild stocks,collected from Laizhuo Bay（LZ）,Haizhuo Bay（HZ）,Xiangshang（XS）,were investigated using 12 microsatellite loci.A total of 115 alleles were detected in 12 microsatellite loci and the number of alleles ranged from 6 to 14（mean 9.583 3）.The expected and observed heterozygosities in 12 loci ranged from 0.765 3 to 0.866 0（mean 0.827 8）and from 0.216 7 to 0.717 6（mean 0.517 5）,respectively.The average polymorphic information content of 12 loci was 0.7051.Mean coefficient of gene differentiation for loci（Fst） was 0.093 0（0.05 Fst 0.15） by F-statistics.UPGMA clustering tree based on D genetic distance showed that Laizhuo Bay stock was more closely related to Xiangshang stock and Haizhuo Bay stock was farthest related to Xiangshang stock.

关 键 词：脊尾白虾 野生群体 微卫星 遗传多样性 

分 类 号：Q785[生物学—分子生物学] S917.4[农业科学—水产科学]