TCF7L2、AKT2、FOXO1基因多态性与2型糖尿病的相关性研究  

The Association Study between Polymorphisms of TCF7L2,AKT2 and FOXO1 Genes and Type 2 Diabetes

作　　者：张沫[1] 樊向维[2] 张捷[1] 刘德敏[1] 

机构地区：[1]天津医科大学代谢病医院、卫生部激素与发育重点实验室,300070 [2]天津市妇女儿童保健中心

出　　处：《天津医药》2012年第12期1188-1192,共5页Tianjin Medical Journal

摘　　要：目的:探讨天津地区汉族人群TCF7L2、AKT2、FOXO1基因多态性与2型糖尿病(T2DM)发生的关系。方法:选取352例T2DM患者(T2DM组)及176例健康体检者(对照组),提取基因组DNA,PCR扩增目的片段后,应用变性高效液相色谱技术检测3种基因的基因型频率,并选取不同峰型进行DNA测序。分析不同基因型频率的组间差异,并对联合基因型进行分析,Logistic回归分析影响T2DM发病的相关因素。结果:2组TCF7L2基因rs12255372、FOXO1基因rs2701891基因型及等位基因频率差异有统计学意义,2组AKT2基因rs11669332基因型及等位基因频率差异无统计学意义。TCF7L2T+/FOXO1T+、TCF7L2G+/FOXO1C+与TCF7L2G+/FOXO1T+在2组间的分布差异有统计学意义。Logistic回归分析结果示糖化血红蛋白及尿素氮升高、TCF7L2T+/FOXO1C+为T2DM的危险因素,高密度脂蛋白胆固醇水平升高为保护因素。结论:携带TCF7L2基因rs12255372位点G/T基因型与FOXO1基因rs2701891位点C/C、C/T基因型者发生T2DM的危险性增大,可能与天津地区汉族人群T2DM的发病有关,且两基因间存在一定协同作用。Objective： To investigate the relationship between the different genetic polymorphisms of transcription factor 7-like 2（TCF7L2）, protein kinase B （AKT2） and forkhead box transcription factor subgroup O1 （FOXO1） and the type 2 diabetes mellitus （T2DM） in Tianjin Han populations. Methods： Three hundred and fifty-two T2DM patients （T2DM group）and 176 healthy subjects （control group）were randomly enrolled in this study. After the genome DNA was extracted, the genotype frequency was detected using the denaturing high performance liquid chromatography. And different peaks were sequenced. Then the differences of different genotype frequencies were analyzed between the groups. The risk factors of T2DM were evaluated using logistic regression. Results： There were significant differences in genotypes and allele frequencies of TCF7L2（rs12255372）and FOXO1 （rs2701891）between two groups. There were no significant differences in the genotype and allele frequency of AKT2（rsl1669332）between two groups. There were significant differences in genotype frequencies of TCF7L2 T＋/ FOXO1T＋,TCF7L2 G＋/ FOXO1 C＋ and TCF7L2 G＋/FOXO1 T＋ between two groups. The logistic regression analysis showed that levels of glycosylated hemoglobin and blood urea nitrogen were increased. TCF7L2 T＋/FOXO1C＋ was the risk factor of T2DM, and high density lipoprotein was a protective factor of T2DM. Conclusion： The G/T genotype in the TCF7L2, C/C and C）T genotypes in the FOXO1 gene significantly contributed to T2DM susceptibility in Tianjin Han populations. A combined effect could be found between TCFTL2 and FOXO1.

关 键 词：糖尿病 2型 多态现象 遗传 TCF7L2 AKT2 FOXO1 天津 汉族 

分 类 号：R541.4[医药卫生—心血管疾病]