替米沙坦对胰岛素抵抗大鼠冠状动脉Rho/ROCK信号通路的影响  

The Effect of Telmisartan on Rho / ROCK Signal Pathway of Coronary Artery in Rat Model of Insulin Resistance

作　　者：丁晓松[1] 李卫萍[1] 沈絮华[1] 武星[1] 李虹伟[1] 

机构地区：[1]首都医科大学附属北京友谊医院心血管中心,100050

出　　处：《天津医药》2012年第12期1230-1233,共4页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(项目编号:30971240);北京市自然科学基金资助项目(项目编号:7092023);教育部高等学校博士点专项科研基金资助项目(项目编号:20091107110005)

摘　　要：目的:观察胰岛素抵抗大鼠和正常大鼠冠状动脉RhoA及ROCK表达水平及替米沙坦对胰岛素抵抗大鼠冠状动脉组织RhoA及ROCK表达水平的影响。方法:30只雄性SD大鼠随机分为3组,对照组予普通饮食;胰岛素抵抗(IR)组给予高果糖饮食+链脲佐菌素(25mg/kg)造模,替米沙坦干预(Tel)组在胰岛素抵抗基础上给予替米沙坦5mg/(kg·d)干预。干预4周后分离冠状动脉采用RT-PCR和Western Blotting法测定各组大鼠冠状动脉组织中RhoA、ROCK1、ROCK2 mRNA和蛋白表达水平。结果:IR组大鼠较对照组RhoA蛋白表达水平降低,ROCK1 mRNA的表达升高。Tel组大鼠RhoA蛋白表达水平较IR组明显升高。IR组及Tel组ROCK2蛋白表达水平较对照组升高,差异有统计学意义,其余组间RhoA mRNA、ROCK1 mRNA、ROCK1蛋白、ROCK2蛋白表达差异均无统计学意义。结论:替米沙坦可部分逆转胰岛素抵抗大鼠冠状动脉RhoA蛋白水平降低,但对RhoA mRNA、ROCK1、ROCK2mRNA及蛋白的表达水平的调节作用不明显。Objective： To observe the role of telmisartan on express levels of RhoA and ROCK signal pathway in coronary artery of rats with insulin resistance. Methods： Thirty male Sprague-Dawley （SD） rats were randomly divided into three groups. The control group was given normal diet （n=10）, insulin resistance group was given high fructose diet plus streptozotocin （25 mg/kg, n=10） and tehnisatan group was given insulin resistance plus telmisartan [5 mg/（kg·d）, n=10]Samples of coronary arteries were isolated for the RT-PCR and Western blot assay to identify RhoA, ROCK1, ROCK2 mRNA and protein expression levels after 4 weeks treatment. Results： Compared with controi group, RhoA protein express level was down regulated, but ROCK1 mRNA express level was up regulated in insulin resistance group. The level of RhoA protein expression was significantly higher in telmisatan group than that of insulin resistance group. Levels of ROCK2 protein expression were significantly higher in insulin resistance group and telmisatan group than that of control group. There were no significant differences in RhoA mRNA, ROCK1 mRNA and ROCK1, ROCK2 protein expressions between insulin resistance group and telmisatan group. Conclusion： Telmisartan can partly reverse the down regulation of RhoA protein in coronary artery of rats with insulin resistance, but no significant effect on RhoA mRNA, ROCK1 and ROCK2 mRNA and protein expressions.

关 键 词：胰岛素抗药性 冠状血管 苯甲酸盐类 苯咪唑类 蛋白质丝氨酸苏氨酸激酶 替米沙坦 RhoA ROCK 

分 类 号：R587.1[医药卫生—内分泌]