代文、阿托伐他汀和匹格列酮对肾上腺髓质素和补体因子H表达的影响  

Effects of Valsartan, Atorvastatin and Pioglitazone on Expressions of Adrenomedullin and Complement Factor H in Mesangial Cells Cultured Under High Glucose Condition

作　　者：钟继娟 缪珩[2] 

机构地区：[1]浙江省中医药大学附属第一医院内分泌科,310006 [2]南京医科大学第二附属医院内分泌科

出　　处：《天津医药》2012年第12期1237-1240,共4页Tianjin Medical Journal

摘　　要：目的:观察代文、阿托伐他汀和匹格列酮对高糖培养的大鼠肾脏系膜细胞转化生长因子(TGF)-β1、细胞外基质(ECM)、肾上腺髓质素(AM)和补体因子H(Cfh)表达的影响,并探讨其作用机制。方法:实验分为低糖对照(LG)、高糖对照(HG)、高糖+代文(HD)、高糖+阿托伐他汀(HL)、高糖+匹格列酮(HP)5组,分别用酶联免疫吸附(ELISA)和放射免疫法(RIA)测定细胞上清TGF-β1、纤维连接蛋白(LN)和Ⅳ型胶原的含量,用逆转录聚合酶链反应(RT-PCR)方法测定TGF-β1mRNA、Cfh mRNA以及AM mRNA的表达情况。结果:高糖状态下培养的肾脏系膜细胞应用代文、阿托伐他汀和匹格列酮干预后,细胞上清TGF-β1、LN和Ⅳ型胶原的含量较HG组显著减低(P<0.01),TGF-β1mRNA、AM mRNA和Cfh mRNA的表达亦较HG组显著减低(P<0.01)。结论:代文、阿托伐他汀和匹格列酮具有一定的肾脏保护作用,其作用机制可能与其抑制TGF-β1的表达有关。药物干预后Cfh和AM的表达也减低,可能与TGF-β的变化有关。Objective： To observe the effects of valsartan, atorvastatin and pioglitazone on the expression of transforming growth factor （TGF）-β1, extracellular matrix （ECM）, adrenomedullin （AM） and complement factor H （Cfh） in mesangial cells cultured under high glucose levels, and the mechanism thereof. Methods： There were five groups in this study including low glucose group （LG）, high glucose group （HG）, high glucose ＋valsartan （HD）, high glucose ＋ atorvastatin （HL） and high glucose ＋pioglitazone （HP）. The enzyme-linked immunosorbent assay （ELISA） and radioimmunoassay （RIA） were used to measure contents of TGF-β1,laminin （LN） and type Ⅳ collagen in cultured mesangial cells under high glucose conditions. RT-PCR was applied to determine the expression of TGF-β1mRNA, Cfh mRNA and AM mRNA. Results： After treatment with valsartan, atorvastatin and pioglitazone, levels of TGF-β1 LN and type IV collagen were significantly decreased in mesangial cells cultured under high glucose （P 〈 0.01）. Expressions of TGF-β1] mRNA, AMmRNA and CfhmRNA and OD value were also decreased after treatment in the cultured mesangial cells under high glucose conditions （P 〈 0.01）. Conclusion： Valsartan, atorvastatin and pioglitazone showed a certain renoprotective functions, which might be related to the inhibition of over-expression of TGF-β1 and extracellular matrix in mesangial cells induced by high glucose. Meanwhile, the expressions of AM and Cfh was reduced, which might also be related to the variation of TGF-β1.

关 键 词：糖尿病肾病 肾小球系膜细胞 吡咯类 噻唑烷二酮类 转化生长因子β1 肾上腺髓质素 补体因子H 缬沙坦 

分 类 号：R589.2[医药卫生—内分泌]