GlyT1、GDNF在面神经后核内侧区的表达  

Expressions of glial glycine transporter 1 and glial cell line-derived neurotrophic factor in the medial area of nucleus retrofacialis

作　　者：李国才 魏巍 郑奇辉[2] 吴中海[3] 龙文飞 肖建斌 招伟贤 

机构地区：[1]广州中医药大学第二附属医院麻醉科,广州510120 [2]兰州大学第二医院麻醉科,兰州730030 [3]南方医科大学生理学教研室,广州510515

出　　处：《中华神经医学杂志》2012年第12期1197-1199,共3页Chinese Journal of Neuromedicine

基　　金：国家自然科学基金（30570670）;广东省医学科研基金（A2008241）

摘　　要：目的观察胶质细胞相关受体甘氨酸转运体1（GlyTl）及胶质细胞源性神经营养因子（GDNF）在延髓面神经后核内侧区（mNRF）的表达情况。方法新生SD大鼠（O～3d）10只，雌雄不拘，按随机数字表法分成对照组、实验组（n=5）。取实验组大鼠离体延髓-脊髓标本制作mNRF“岛”，对照组大鼠取三叉神经核周围脑组织。荧光定量RT-PCR测定GlyTl、GDNF在mNRF“岛”和三叉神经核周围脑组织的表达。结果实验组mNRF“岛”区Gly、GDNF基因的表达量均高于对照组三叉神经核周围脑组织中的基因表达量，差异有统计学意义（t=5．112，P=0．000；t=3．532，P=-0．003）。结论GlyTl、GDNF在mNRF存在高表达，但这两种物质在节律性呼吸发生及调控过程中的作用及与神经元间的具体联系尚有待进一步研究。Objective To investigate the mRNA expressions of glial glycine transporter 1（GlyT1） and glial cell line-derived neurotrophic factor （GDNF） genes in the medial area of nucleus retrofacialis （mNRF）. Methods Ten neonatal SD rats （aged 0-3 d） were randomly divided into control and experimental groups （n=5）; the mNRF ＂islands＂ were isolated from the brainstem slices in rats of the experimental group, while trigeminal nucleus surrounding brain tissues were chosen in rats of the control group. GlyT1 and GDNF gene expressions were detected using real time quantitative PCR （RT-PCR）. Results The expressions of GlyT1 and GDNF genes in the mNRF ＂island＂ were both significantly higher than those in the controls （t=5.112, P=0.000; t=3.532, P=-0.003）. Conclusion The mRNA expressions of GlyT1 and GDNFgenes are high in the mNRF; however, the roles of them in generation and modulation in rhythmical respiration and the mechanisms between neurons and both GlyT1 and GDNF should be further studied.

关 键 词：胶质细胞 面神经后核内侧区 呼吸 

分 类 号：R332.3[医药卫生—人体生理学]