绞股蓝总皂甙对慢性脑缺血性白质氧化性损伤的保护作用  

Protective effect of gypenoside on oxidative damage of the white matter in rats after chroniccerebral hypoperfusion

作　　者：张广林 王本瀚 熊家锐 郭效东 张长远 高国栋[2] 

机构地区：[1]解放军第一五三医院神经外科,郑州450042 [2]第四军医大学唐都医院神经外科,西安710038

出　　处：《中华神经医学杂志》2012年第12期1219-1224,共6页Chinese Journal of Neuromedicine

摘　　要：目的探讨绞股蓝总皂甙对慢性脑缺血大鼠脑白质氧化性损伤的保护作用以及对脑缺血大鼠认知功能的影响。方法将57只成年雄性SD大鼠按随机数字表法分成假手术组（n=12）、模型组（n=15）、绞股蓝总皂甙200mg组（n=15）、绞股蓝总皂甙400mg组（n=15），后3组采用双侧颈总动脉结扎法制备慢性脑缺血模型，且在造模后3h分别将等量生理盐水，200mg／kg、400mg／kg绞股蓝总皂甙溶液灌胃，1次／d，持续33d。应用Morris水迷宫实验测试各组大鼠空间学习与记忆能力的改变，酶联免疫吸附法（ELISA）N0定大鼠胼胝体、视束内超氧化物歧化酶（SOD）活性、丙二醛（MDA）含量，免疫组化染色用8-羟基脱氧鸟苷（8-OHdG）抗体测定中枢神经细胞的氧化损伤水平。结果与模型组相比，绞股蓝总皂甙400mg组大鼠的逃避潜伏期明显缩短及在原平台象限的游泳时间明显延长，差异有统计学意义（P＜0．05）。与假手术组比较，模型组大鼠胼胝体及视束内MDA含量明显增加，SOD活性明显降低，差异有统计学意义（P〈O．05）。与模型组相比，绞股蓝总皂甙400mg组MDA含量明显降低，SOD活性明显升高，8-OHdG阳性细胞数明显减少，差异有统计学意义（P〈0．05）。与模型组相比，绞股蓝总皂甙200mg组SOD活性、MDA含量及8-OHdG阳性细胞数差异无统计学意义（P〉0．05）。结论绞股蓝总皂甙能有效改善慢性脑缺血大鼠脑白质氧化性损伤，提示其可能是一种有效的抗痴呆药物，但其作用的确切机制还有待进一步研究。Objective To investigate the protective effect of gypenoside （GP） on oxidativedamage of the white matter in rats after chronic cerebral hypoperfusion and on its alterations of cognitive function. Methods A total of 57 male SD rats were randomly assigned to 4 groups： sham-operated group （n=12）, vehicle group （n=15）, 200 mg/kg GP treatment group （n=15） and 400 mg/kg GP treatment group （n=15）; chronic cerebral hypo-perfused models in the later 3 groups were established by permanent bilateral common carotid artery occlusion （2-VO）. The dosage volume of all groups was 10 mL/kg; 3 h after the surgery, all rats orally received the initial administration of 400 mg/kg or 200 mg/kg GP dissolved in saline solution or matched volume of normal saline daily for a consecutive 33 d according to the above-mentioned experimental plan. Spatial learning and memory were assessed using Morris water maze test. Following behavioral tests, the activities of superoxide dismutase （SOD） and changes of malondialdehyde （MDA） level in the corpus callosum and optic tracts were measured by ELISA. The oxidative central nerve cell damages were assessed by immunohistochemical staining for 8-hydroxy-2＇-deoxygnanosine （8-OHdG）. Results Rats of the 400 mg/kg GP treatment group spent significantly fewer time in finding the platform, but significantly longer time spending in the platform region as compared with those of the vehicle group （P〈0.05）. As compared with those in rats of the sham-operated group, the SOD activity was markedly reduced and MDA content was significantly increased in rats of the vehicle group （/9〈0.05）. Rats of the 400 mg/kg GP treatment group had decreased MDA content, increased SOD activity and decreased 8-OHdG level as compared with rats of the vehicle group （P〈0.05）; however, 200 mg/kg GP treatment group had no such significant effects as compared with those of the vehicle group （P〉0.05）. Conclusion GP can ameliorate the oxidative damage in the corpus callosum and

关 键 词：绞股蓝总皂甙 脑缺血 脑白质损伤 氧化应激 空间学习与记忆 

分 类 号：R285.5[医药卫生—中药学]