小鼠慢性病毒性肝炎诱发肝脂肪变性模型的构建  

Establishment of a murine model of hepatic steatosis induced by chronic viral hepatitis

作　　者：甘露[1] 张哲[1] 郭进强[1] 谢茜[1] 蒙子君[1] 万为人[1] 罗炳德[1] 

机构地区：[1]南方医科大学公共卫生与热带医学学院,广东广州510515

出　　处：《南方医科大学学报》2012年第12期1722-1726,共5页Journal of Southern Medical University

基　　金：国家自然科学基金(31070687);广东省自然科学基金(9451051501002535);南方医科大学公共卫生与热带医学学院学生课外科研项目(GWXS20110231)~~

摘　　要：目的用小鼠肝炎病毒MHV-A59感染C57BL/6小鼠,建立慢性病毒性肝炎诱发肝脂肪变性的动物模型。方法 C57BL/6小鼠随机分为对照组、高脂组、病毒组和高脂病毒组。实验第13周末取血清检测MHV抗体、脂质和转氨酶;小鼠肝脏冰冻切片进行油红O染色,石蜡切片进行HE染色,观察各组肝组织改变状况。结果病毒组与高脂病毒组血清MHV抗体强阳性;与对照组相比,3个处理组的AST、ALT明显增高,TC和LDL-C均有不同程度增高;病毒组与高脂病毒组的肝组织表现出慢性病毒性肝炎的病理特征,肝细胞内脂质蓄积增多。结论通过感染MHV-A59病毒,成功建立了慢性病毒性肝炎诱发肝脂肪变性的动物模型,为进一步研究慢性病毒性肝炎诱发肝脂肪变性的机制提供了基础。Objective To establish a animal model of hepatic steatosis induced by chronic viral hepatitis in C57BL/6 mice. Methods C57BL/6 mice were randomly assigned to control group, high-fat diet group, mouse hepatitis virus strain A59 （MHV-A59） virus infection group, and high-fat diet plus virus infection group. At 13 weeks of the experiment, serum samples were collected to detect MHV antibodies and transaminase and lipid levels. The hepatic pathologies of the mice were examined with Oil red O staining of the frozen sections the and HE staining of paraffin-embedded sections. Results The mice in the two virus infection groups showed strong positivity of MHV antibodies in the serum. Compared with the control group, the mice in high-fat diet group and the two virus infection groups had significantly increased AST and ALT levels with also elevated TC and LDL-C levels. The two virus infection groups both exhibited obvious pathologies in the liver characteristic of chronic viral hepatitis with increased lipid accumulation in the hepatocytes. Conclusion We have successfully established a mouse model of hepatic steatosis induced by chronic viral hepatitis, which provides the basis for further study of the disease mechanism.

关 键 词：MHV-A59 慢性病毒性肝炎 肝脂肪变性 动物模型 

分 类 号：R512.6[医药卫生—内科学] R-332[医药卫生—临床医学]