高迁移率族蛋白B1协同白介素-1β可增加人气道上皮细胞白介素-8的表达  

High-mobility group box protein 1 in synergy with interleukin-1β promotes interleukin-8 expression in human airway epithelial cells in vitro

作　　者：张丹丹[1] 赵海金[1] 周丽芹[1] 宋甲富[1] 董航明[1] 邹飞[2] 蔡绍曦[1] 

机构地区：[1]南方医科大学南方医院呼吸与危重症医学科,广东广州510515 [2]南方医科大学公共卫生与热带医学学院,广东广州510515

出　　处：《南方医科大学学报》2012年第12期1764-1767,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(81270087;81270089;30971328);国家重点基础研究发展计划(973计划)(2012CB518203);高等学校博士学科点专项科研基金(20094433110011);南方医院院长基金(2011Z011)~~

摘　　要：目的探讨损伤相关分子模式分子高迁移率族蛋白B1(HMGB1)单独或联合白介素-1β(IL-1β)对人气道上皮细胞(16HBE,A549)白介素-8(IL-8)表达水平的影响。方法重组人HMGB1与IL-1β复合物的制备:一定浓度的HMGB1与IL-1β混匀后于4℃冰箱静置16 h。实验分组:对照组,HMGB1-100 ng/ml,IL-1β-10 ng/ml,HMGB1-25 ng/ml+IL-1β-10 ng/ml,HMGB1-100 ng/ml+IL-1β-10 ng/ml处理气道上皮细胞24 h,四唑盐(MTT)法检测不同刺激组对气道上皮细胞活力的影响,实时荧光定量PCR检测细胞IL-8mRNA表达水平,ELISA检测细胞培养上清中IL-8的浓度。结果在不影响细胞活力的情况下,HMGB1-100 ng/ml对气道上皮细胞IL-8的表达水平无显著影响,但与IL-1β形成复合物后,可协同IL-1β显著增加16HBE和A549 IL-8的表达和释放(P<0.05),并具有浓度依赖趋势。结论 HMGB1可协同IL-1β促进气道上皮细胞IL-8的表达,可能为HMGB1参与气道中性粒细胞炎症提供新的证据。Objective To test the effect of high-mobility group box protein 1 （HMGB1） alone or in synergy with interleukin-1β （IL-1β） on the expression of IL-8 in human airway epithelial cells in vitro. Methods Human airway epithelial 16HBE and A549 cell lines were incubated with HMGB1 （100 ng/ml） in the absence or presence of IL-1β （10 ng/ml） for 24 h, and the changes of IL-8 mRNA and protein expressions were assessed using quantitative PCR and enzyme-linked immunosorbent assay （ELISA）. Results In the two human airway epithelial cell lines, HMGB1 alone did not produce obvious effect on the expression of IL-8, but in the presence of IL-1β, HMGB1 caused a significant increase of IL-8 expressions at both the mRNA and protein levels. Conclusion HMGB1 in synergy with IL-1β increases the expression of IL-8 in human airway epithelial cells, which provides new evidence that HMGB1 contributes to neutrophilic airway inflammation by regulating IL-8 expression.

关 键 词：损伤相关分子模式分子 HMGB1 气道上皮细胞 气道炎症 白细胞介素-8 

分 类 号：R562.25[医药卫生—呼吸系统]