罗格列酮对RAW264.7巨噬细胞源性泡沫细胞内胆固醇含量及SR-BⅠ表达的影响  

Effects of rosiglitazone on cholesterol contents and scavenger receptor class B type I expression in RAW264.7 foam cells

作　　者：徐芳[1] 蒙颖[1] 王志禄[2] 李万玲[1] 贾军正[1] 郭文芬[1] 谢宛霞[1] 胡海英[1] 胡旭堂[1] 

机构地区：[1]兰州大学第一临床医学院,甘肃兰州730000 [2]兰州大学第一医院心内科,甘肃兰州730000

出　　处：《南方医科大学学报》2012年第12期1792-1795,共4页Journal of Southern Medical University

基　　金：甘肃省自然科学基金(1010RJZA122);甘肃省卫生行业科研计划管理项目(GWGL2010-16)

摘　　要：目的通过观察罗格列酮对RAW264.7泡沫细胞内胆固醇含量和酰基辅酶A-胆固醇酰基转移酶1(ACAT-1)及B族Ⅰ型清道夫受体(SR-BⅠ)表达的影响,探讨罗格列酮抗动脉粥样硬化的作用机制。方法以RAW264.7巨噬细胞源性泡沫细胞为研究对象,分为空白对照组、泡沫细胞组和罗格列酮组,用不同浓度的罗格列酮(5、10和20μmol/L)对泡沫细胞进行干预。采用油红O染色观察泡沫细胞形成,胆固醇氧化酶法检测泡沫细胞内胆固醇含量的变化;Western blotting技术检测泡沫细胞表面ACAT-1及SR-BⅠ的表达。结果与对照组相比,泡沫细胞组内总胆固醇及游离胆固醇含量升高(P<0.01)、ACAT-1表达增加(P<0.05)、SR-BⅠ表达略有增加(P>0.05);与泡沫细胞组相比,不同浓度罗格列酮干预组细胞内总胆固醇及游离胆固醇含量下降(P<0.05)、ACAT-1表达下降(P<0.05)、SR-BⅠ表达升高(P<0.05),呈现出一定的浓度依赖性。结论罗格列酮可通过下调ACAT-1的表达和上调SR-BⅠ的表达来降低泡沫细胞内胆固醇含量,从而发挥抗动脉粥样硬化作用。Objective To observe the effect of rosiglitazone on the content of cholesterol and expressions of Acy-conzyme A： cholesterol acytransferase 1 （ACAT-1） and scavenger receptor class B type I （SR-BI） in RAW264.7 macrophage-derived foam cells and explore the anti-atherosclerotic mechanism of rosiglitazone. Methods RAW264.7 macrophages were incubated with oxidized low-density lipoproteins （ox-LDL） or with both ox-LDL and rosiglitazone （5, 10, or 20 μmol/L）. Oil red O staining was used to observe the formation of foam cells, and cholesterol oxidase was used to determine the content of cellular cholesterol contents. Western blotting was used observe the expressions of ACAT-1 and SR-BI in RAW264.7 foam cells. Results Compared with the control cells, RAW264.7 macrophage-derived foam cells showed significantly increased contents of total cholesterol and free cholesterol （P〈0.01） and ACAT-1 expressions （P〈0.05） with mildly increased SR-BI expression （P〉0.05）. Rosiglitazone treatments significantly lowered the contents of total cholesterol and free cholesterol （P〈0.05）, decreased the expression of ACAT-1 （P〈0.05）, and increased SR-BI expression （P〈0.05） in the foam cells in a＇ dose-dependent manner. Conclusion Rosiglitazone can decrease the contents of total and free cholesterol, down-regulate ACAT-1 expression and up-regulate SR-BI expression in the foam cells produce the anti-atherosclerotic effect.

关 键 词：罗格列酮 泡沫细胞 胆固醇 酰基辅酶A-胆固醇酰基转移酶1 B族Ⅰ型清道夫受体 

分 类 号：R341[医药卫生—基础医学]