酸的种类、pH和温度对酸性纤维素酶活力的影响  

Effects of acids,pH and temperature on acid cellulase activity

作　　者：刘幸乐[1] 姚继明[1] 吴远明[1] 

机构地区：[1]河北科技大学纺织服装学院,河北石家庄050018

出　　处：《印染助剂》2012年第12期11-14,共4页Textile Auxiliaries

基　　金：石家庄市科技局科技支撑项目(11119661A)

摘　　要：以8种酸(甲酸、冰醋酸、羟基乙酸、乳酸、柠檬酸、酒石酸、氨基乙酸和氨基磺酸)配制成pH=5.0的缓冲液,测定酸性纤维素酶在各缓冲液中的活力.同时以冰醋酸作为pH调节剂,采用中心合成设计法,分析和优化pH和温度对酶活力的影响,得出线性回归方程和优化值.结果表明,在pH=5.0的条件下,乳酸和酒石酸为酸剂的酶活力高于冰醋酸,羟基乙酸、甲酸和柠檬酸比冰醋酸略低,而氨基乙酸和氨基磺酸明显低于冰醋酸;采用冰醋酸调节pH,酶活力随着温度的升高和pH的降低而增加,pH影响的显著性要大于温度.酸性纤维素酶在49.8℃,pH=4.8可以实现最佳的活力.Eight kinds of acids （formic acid, acetic acid, glycolic acid, lactic acid, citric acid, tartaric acid, glycine and aminosulphonic acid） were used as buffer solutions with pH=5.0. The acid cellulase activities were measured individually in these buffer solutions. Meanwhile using acetic acid as pH regulator, the effects of pH and temperature on cellulose activity were analyzed and optimized by central composite design to obtain linear regression equatian and optimal factors. The results showed that tartaric acid and lactic acid exhibited higher activity than acetic acid at pH 5.0, glycolic acid, formic acid and citric acid gave little lower activity compared with acetic acid, while the glycfne, aminosulphonic acid gave much lower cellulase activity than acetic acid. Ap- plying acetic acid to adjust pH, cellulase activity increased with the rising of temperature and the decline of pH. pH showed more significance than temperature, the optimum performance of activity was at 49.8 ℃, pH= 4.8.

关 键 词：酸性缓冲液 纤维素酶活力 线性回归 中心合成设计法 

分 类 号：TQ426.97[化学工程]