人类胚胎耳蜗抗原相关抗体检测及其意义  

Test of human embryo cochlear antibody and its effect

作　　者：张建国[1] 黄振先[1] 张子学 严小玲[1] 陈一心 

机构地区：[1]广州医学院附属第二医院耳鼻咽喉科,广东广州510260

出　　处：《中国耳鼻咽喉颅底外科杂志》2000年第2期97-99,共3页Chinese Journal of Otorhinolaryngology-skull Base Surgery

摘　　要：目的　探讨自身免疫性耳聋的机理及有效的诊断方法。方法　取胎龄为 4～ 7月的膜迷路制备人类胚胎耳蜗抗原 (HumanembryocochlearAg ,HEC -Ag)。取HEC -Ag液为兔子做皮内注射 ,留取血清作阳性血清对照。健康兔血清作阴性血清对照。收集 76例患者的血清为耳聋组 ,34例正常健康人血清为对照组。对血清进行HEC -Ab的检测。结合临床疗效分析。结果　耳聋组光密度值为 0 .16 4± 0 .0 58,对照组光密度值为 0 .0 6 3± 0 .0 31(P<0 .0 1)。在耳聋组中 13例阳性 (17.1% ) ,2例强阳性 (2 .6 % ) ,阳性率为 19.7%。在对照组中 2例阳性 ,阳性率为 5.9% (P <0 .0 1)。结论　提示HEC -Ab的检测可作为自身免疫性耳聋诊断的初步筛选方法。Objective To study the mechanism and effective diagnosis methods of autoimmune sensorineural hearing loss. Methods The membranous labyrinth of human embryo (4～7 months) was taken out. Human Embryo Cochlear Antigen (HEC-Ag) was prepared. The rabbit was injected with HEC-Ag. A month later, its blood was taken as positive control serum. The blood of a healthy rabbit was taken as negative control serum. Seventy-six patients formed the sensorineural hearing loss group and 34 healthy people were in the control group. The HEC-Ab in the serum was tested. The mechanism and effective diagnosis methods of autoimmune sensorineural hearing loss were discussed in combination with the clinical results. Results The absorbency (A) of positive control serum in the rabbit was 0.103±0.034 and the A of negative control serum in the rabbit was 0.077 ± 0.012 . The A in the experimental group was 0.164 ± 0.058 and in the control group it was 0.063 ± 0.031 (P< 0.01 ). Thirteen cases were positive in the experimental group ( 17.1% ) and 2 were strongly positive ( 2.6% ). The positive rate was 1 9.7% . Two cases were positive in the control group ( 5.9% ) (P< 0.01 ). Conclusion The HEC-Ab test is a filtration method in the diagnosis of autoimmune sesorineural hearing loss.

关 键 词：自身免疫性耳聋 HEC-Ag 血清诊断 耳蜗抗原 

分 类 号：R764.430.4[医药卫生—耳鼻咽喉科]