丹酚酸B联合骨髓间充质干细胞移植对大鼠脊髓损伤修复作用研究  

Study on the restorative effect of salvianolic acid B combined with bone mesenchymal stem cells transplantation on tat spinal cord injury

作　　者：王飞[1] 张晓君[1] 岳冀[1] 于大鹏[1] 赵廷宝[1] 

机构地区：[1]济南军区总医院脊髓修复科,250031

出　　处：《环球中医药》2012年第11期804-807,共4页Global Traditional Chinese Medicine

摘　　要：目的探讨丹酚酸B联合骨髓间充质干细胞(BMSCs)移植对大鼠脊髓损伤(SCI)模型的修复作用,以期为脊髓损伤修复提供新的治疗方案。方法采用改良Allen打击法制作SCI模型,利用BBB评分法评价大鼠神经功能恢复情况,并用HE法和免疫组化法分别对脊髓组织病理形态及GFAP和NGF的表达情况进行分析。结果术后大鼠后肢运动功能逐渐改善,C、D组BBB评分明显高于B组(P<0.01)。脊髓组织病理切片显示受损脊髓较模型组有明显修复,胶质细胞增生活跃,损伤周围可见相对完整的神经细胞。从免疫组化的结果来看,各组大鼠的GFAP和NGF均有表达,A组GFAP和NGF阳性细胞面积高于B、C、D组;C、D组高于B组(P<0.01);D组又高于C组(P<0.05)。结论 BMSCs移植对损伤后的大鼠脊髓有保护作用,丹酚酸B能协同BMSCs促进对大鼠脊髓损伤的修复。Objective In order to provide a new treatment for repairing spinal cord injury,we explore the restorative effect of salvianolic acid B combined with bone mesenchymal stem cells（BMSCs） transplantation on rat spinal cord injury（SCI） model.Methods Modified Allen punch method is adopted to make SCI model.The recovery condition of the rat nerve function is evaluated by BBB scores.HE method and immunohistochemistry are used to separately analyze the Spinal cord tissue pathological morphology and the expression of GFAP and NGF.Results After operation the rat＇s hindlimb motor function gets better gradually with the BBB scores of group C and D much higher than that of group B（P0.01）.The spinal cord tissue pathological section shows the obvious restoration of injured spinal cord compared with the model group,active proliferation of gliocyte and relatively completed nerve cells in the damaged surrounding.From the results of immunohistochemistry,the rat GFAP and NGF in each group are all expressed.The positive cells area of GFAP and NGF in group A is larger than that in group B,C and D;in group C and D it is larger than that in group B（P0.01）;in group D it is larger than that in group C（P0.05）.Conclusion BMSCs transplantation has protective effect on rat spinal cord,and salvianolic acid B can cooperate with it to promote the recovery of rat spinal cord injury.

关 键 词：脊髓损伤 丹酚酸B 骨髓间充质干细胞 损伤修复 

分 类 号：R285.5[医药卫生—中药学]