烧伤病人痂下水肿液对中性粒细胞膜表面CD11/CD18表达的影响  

Effect of subeschar tissue fluid from burn patients on the expression of CD11/CD18 on PMN in vitro

作　　者：李志清[1] 周一平[1] 荣新洲[1] 陈文[1] 王振岳[1] 

机构地区：[1]第一军医大学南方医院烧伤科,广东广州510515

出　　处：《第一军医大学学报》2000年第3期222-223,共2页Journal of First Military Medical University

基　　金：军队九五科技攻关项目

摘　　要：目的 了解烧伤病人痂下水肿液（STF）对中性粒细胞（PMN）活化及CD11/CD18表达的影响。方法 应用流式细胞术动态检测严重烧伤病人STF 刺激健康人PMN后PMN膜表面CD11a/CD18和CD11b/CD18的表达以及STF与PMN同孵育后培养液中髓过氧化物酶（MPO）变化。结果 健康人PMN在STF刺激1 h后，培养液中MPO活性即迅速升高并在24 h内保持此高水平，同时，PMN膜表面CD11a/CD18和CD11b/CD18也迅速升高达峰值并在24 h内保持此高水平。结论 STF可活化PMN并刺激PMN表达CD11a/CD18和CD11b/CD18，STF对烧伤后PMN致组织损害可能起重要作用。Objective To investigate the effect of changes in CD11/CD18 expressions on polymorphonuclear neutrophils (PMNs) in response to subeschar tissue fluid (STF) obtained from burn patients. Methods The expression of CD11a/CD18 on the membrane of STF-treated PMN from normal subjects and CD11b/CD18 were determined by flow cytometry, and the activity of myeloperoxidase (MPO) in the media was determined. Results In response to STF stimulation, the expression of CD11a/CD18 and CD11b/CD18 on the membrane of PMN increased, reaching its peak at 1 h, and maintained during the following 24 h. The activity of MPO in the media underwent almost the same process. Conclusion STF can activate the PMNs and stimulate the expression of CD11/CD18 on the membrane of PMNs.

关 键 词：烧伤 痂下水肿液 中性粒细胞 CD11/CD18 

分 类 号：R644[医药卫生—外科学]