骨碎补总黄酮对大肠杆菌脂多糖诱导的急性肾衰竭大鼠ICAM-1基因表达的影响  

Effect of Flavonoids of Rhizoma Drynariae on the Expression of ICAM-1 in Rats with E.coli LPS-induced Acute Renal Failure

作　　者：罗颖[1] 蒋文功[1] 崔淑兰[1] 

机构地区：[1]广东药学院,广东广州510006

出　　处：《河北医学》2013年第1期12-16,共5页Hebei Medicine

基　　金：广东省医学科研基金项目:编号A2007303

摘　　要：目的:探究骨碎补总黄酮对大肠杆菌脂多糖(LPS)诱导的急性肾衰竭大鼠ICAM-1基因表达的影响。方法:将80只雄性三月龄的大鼠平均分为四组,其中对照组水灌胃7d后腹腔注射生理盐水,LPS组则水灌胃7d后腹腔注射30mg/kg的LPS,而AFFDR组用骨碎补总黄酮灌胃,LPS+AF-FDR组则先使用骨碎补总黄酮灌胃7d,然后腹腔注射LPS,2h后接着用骨碎补总黄酮灌胃。观察测定并比较各组大鼠血清中的肌酐值,同时比较各组大鼠肾脏病理检查结果,观察比较ED-1阳性巨噬细胞的浸润,并且采用半定量化的RT-PCR方法检测骨碎补总黄酮对肾组织ICAM-1基因表达的影响。结果:LPS可以造成大鼠Scr的显著升高和肾小管病理改变,而AFFDR可以有效降低LPS的上述影响;同时对照组相比,LPS显著提高了肾组织ED-1阳性巨噬细胞浸润以及ICAM-1 mRNA的表达,但是LPS+AFFDR组在这两个指标上又显著低于LPS组。结论:AFFDR可以有效降低LPS对肾小管超微结构造成的病理损害以及大鼠血清肌酐的含量,同时还可以对由LPS诱导的肾组织ED-1巨噬细胞浸润和ICAM-1的表达产生较强的抑制作用。Objective： To explore the effect of flavonoids of rhizoma drynariae on the expression of ICAM-1 in rats with E.coli lipopolysaccharide-induced acute renal failure.Method： 80 male three months-old rats were divided into four groups.The control group was intraperitoneally injected saline after water gavage 7days.LPS group was intraperitoneally injected 30mg/kg LPS after water gavage 7days,while the AFFDR group was given a gavage of flavonoids of rhizoma drynariae.LPS ＋ AFFDR group was firstly given a gavage of flavonoids of rhizoma drynariae for 7 days,then was intraperitoneally injected LPS,given a gavage of flavonoids of rhizoma drynariae two hours later.We observed and compared the serum creatinine values.Each group was compared in rat kidney pathological.We also compared the ED-1 positive macrophage infiltration.and used semi-quantitative RT-PCR method to detect the effect of flavonoids of rhizoma drynariae on the expression of ICAM-1.Result： LPS significantly increased rats Scr and renal tubule pathological changes,while AFFDR can effectively reduce the above-mentioned effects of the LPS.Compared with the control group,LPS significantly improved ED-1 positive macrophage infiltration and ICAM-1 mRNA expression in the renal tissue,but the indexes of LPS ＋ AFFDR group were significantly lower than the LPS group.Conclusion： AFFDR can effectively reduce the pathological damage caused by LPS on renal tubular ultrastructure and serum creatinine level,can also inhibit ED-1 macrophage infiltration and ICAM-1 expression induced by LPS.

关 键 词：骨碎补总黄酮 急性肾衰竭 LPS ICAM-1 

分 类 号：R285.5[医药卫生—中药学]