硼酸盐聚合物层数对表面等离子共振谱葡萄糖浓度测量的影响  

Effect of Borate Polymer Layers on Measurement of Glucose Concentration by SPR

作　　者：栗大超[1] 杨佳[1] 伍鹏[1] 朱芮[1] 王博[1] 林园[1] 徐可欣[1] 

机构地区：[1]天津大学精密测试技术及仪器国家重点实验室,天津300072

出　　处：《光谱学与光谱分析》2013年第1期142-146,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(30800239;61176107);天津市科技支撑计划重点项目(11ZCKFSY01500)资助

摘　　要：在表面等离子共振谱(surface plasmon resonance,SPR)葡萄糖浓度测量中,引入一种全新的能够特异性吸附葡萄糖分子的硼酸盐聚合物PAA-ran-PAAPBA,实现了葡萄糖浓度的特异性检测。采用层层自组装的方法分别在SPR传感器表面绑定6层和12层硼酸盐聚合物,研究了聚合物层数对葡萄糖浓度测量效果的影响。分别在浓度范围1～10mg.dL-1(浓度间隔Δ=1mg.dL-1)、10～100mg.dL-1(浓度间隔Δ=10mg.dL-1)、100～1 000mg.dL-1(浓度间隔Δ=100mg.dL-1)内进行实验,实验数据采用二次曲线进行拟合,得到折射率差值ΔRU和葡萄糖浓度之间的拟合度。实验结果显示在前两个较小葡萄糖浓度范围内绑定有12层聚合物传感器的测量效果要明显好于6层聚合物,在第三个较大葡萄糖浓度范围内聚合物层数的影响并不明显,表明在小浓度范围内增加聚合物绑定层数可以改善葡萄糖测量效果。A new borate polymer PAA-ran-PAAPBA that can specifically adsorb glucose was introduced in the glucose measurement based on surface plasmon resonance,and the high-precision specific detection of glucose concentration was realized.Six layers and twelve layers of borate polymer were respectively bound onto the SPR sensors through the layer-by-layer self-assembly binding method,and the effect of different layers of borate polymer on the glucose surface plasmon resonance measurement was studied.The experiment was conducted in the concentration range of 1~10 mg·dL-1（interval Δ=1 mg·dL-1）,10~100 mg·dL-1（interval Δ=10 mg·dL-1）,and 100~1 000 mg·dL-1（interval Δ=100 mg·dL-1）,experiment data was fitted by quadric curve and the fitting degree of refractive index difference ΔRU and glucose concentration was obtained.Results showed that the 12-layer-polymer sensor was better than the 6-layer-polymer sensor in the first two smaller ranges,and the measuring result was not significantly affected by layers in the third range,indicating that for the small concentrations increasing polymer layer can dramatically improve the measurement.

关 键 词：葡萄糖 浓度测量 表面等离子共振 硼酸盐聚合物 层层自组装 

分 类 号：O443[理学—电磁学]