肾小管间质纤维化中成纤维细胞的转化生长因子β1及其Ⅰ型受体蛋白及基因表达检测  

Detection of TGF-β_1 and its type Ⅰ receptor in fibroblasts in tubulointerstitial fibrosis

作　　者：孙锁柱[1] 徐曼[1] 邹万忠[1] 

机构地区：[1]北京医科大学病理学系

出　　处：《临床与实验病理学杂志》2000年第2期134-136,共3页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家自然科学基金资助课题!(No 3 9770 2 93 )

摘　　要：目的 :研究腺嘌呤致大鼠肾小管间质纤维化模型的肾组织成纤维细胞中转化生长因子 β1(TGF β1)及其Ⅰ型受体的表达。方法 :腺嘌呤灌胃法建立大鼠肾小管间质纤维化模型 ,由模型肾组织培养肾间质成纤维细胞 ,应用免疫组化、逆转录PCR分别检测TGF β1及其Ⅰ型受体蛋白及mRNA的表达。结果 :成纤维细胞TGF β1、TGF β1I型受体免疫组化阳性 ;逆转录PCR可见 2 94bp的TGF β1及 34 5bp的TGF β1的Ⅰ型受体mRNA特异扩增带。结论 :在肾小管间质纤维化进程中 ,肾间质成纤维细胞是TGF β1的一个来源 ,而且也是其效应细胞 ,从而在TGF β1的作用下出现增生和合成细胞外基质的效应。Purpose To investigate the expression of TGF β 1 and its type I receptor in fibroblasts in the renal tubulointerstitial fibrosis animal model. Methods The tubulointerstitial fibrosis model was established in Wistar rats by gastroperfusion of excessive adenine,and then to culture the renal interstitial fibroblasts. The expressions of protein and mRNA for TGF β 1 and its type I receptor were examined utilizing immunohistochemistry, and reverse transcription polymerase chain reaction(RT PCR) Results Immunohistochemistry showed positivity of TGF β 1 and its type I receptor in fibroblasts. The 294bp and 345bp special RT PCR productions for TGF β 1 and its type Ⅰ receptor mRNA can also be detected in the cultured fibroblasts. Conclusion Fibroblast is a origin and effective cell for TGF β 1 in tubulointerstitial fibrosis

关 键 词：肾小管间质纤维化 Ⅰ型受体 TGF-Β1 

分 类 号：R692.602[医药卫生—泌尿科学]