改良的Ellman's法测定小鼠肝脏还原型谷胱甘肽含量  

Determination of the GSH Contents of Mice Liver by Reformed Ellman's Method

作　　者：黎煌久[1] 陈焕昭[1] 刘幸平[1] 

机构地区：[1]汕头大学医学院生化教研室,汕头515031

出　　处：《汕头大学医学院学报》2000年第2期16-16,19,共2页Journal of Shantou University Medical College

摘　　要：目的 :观察辅酶Q(CoQ10 )对急性肝损伤的保护作用。方法 :采用改良的Ellman s法测定小鼠肝脏还原型谷胱甘肽(GSH)的含量。结果 :CoQ10 高、低剂量组 ,肝脏GSH含量均较生理盐水 (NS)对照组高 ,其差异有显著和极显著性意义 (P <0 0 5和P <0 0 1)。结论 :CoQ10 能减少超量醋氨酚 (AAP)Objective:To observe the protective effects of coenzyme Q 10 on acute hepatic injury.Method:The GSH contents of mice liver were determined by reformed Ellmans method.Results:The GSH contents of higher(25 0mg/kg)and lower(12 5mg/kg)CoQ 10 dosage groups were higher than those of the NS controls.The satisfactary tests showed significant differences and extremely significant differences(P<0 05 and P<0 01).Conclusion:The CoQ 10 can reduced the consumption of the over dosage of AAP on hepatic GSH.

关 键 词：谷胱甘肽 Ellman's 辅酶Q10 急性肝损伤 

分 类 号：R657.304[医药卫生—外科学]