丙交酯乙交酯共聚物的毒性试验及其含左旋18-甲基炔诺酮微球对鼠的抗生育作用研究  

Studies on the Toxicity of Polymer of Glycolide and Lactide(PLGA)and the Anti-fertility Effect of Levonorgestrel (LNG)

作　　者：金爱华[1] 张寅恭[1] 焦捷军 朱家惠 周芝芳[1] 陶沁[1] 

机构地区：[1]浙江省医学科学院计划生育研究所,杭州310013

出　　处：《生物医学工程学杂志》2000年第2期125-128,共4页Journal of Biomedical Engineering

基　　金：国家计生委资助!93-0-30;浙江省卫生厅基金!95028

摘　　要：对可生物降解聚合物丙交酯乙交酯共聚物（PLGA9:1）进行了急性全身毒性、眼结膜刺激、肌肉刺激、热源、溶血、过敏等试验和含LNG的PLAG微球注射剂对小鼠和大鼠的抗生育作用试验。结果表明：PLGA无毒、对眼结膜和肌肉无刺激性、无热源作用、无溶血作用、无致敏作用，具有良好的生物相容性。小鼠皮下注射PLGA－LNG微球5、10、20ms／kg，避孕天数分别为11．5、34．6、81．2d，大鼠肌肉注射PLGA－LNG微球13．6、19．8、30mg／kg，对大鼠动情周期抑制为76．3、104．1、131．8d，两者均较注射同等剂量的LNG微晶组动物作用明量增强。提示PLGALNG微球有长效抗生育作用。PLGA(9: 1 ),a chemical degraded from biomaterials,has been subjected to the tests of acute toxicity,irritations on conjunctiva and muscle,haemolysis in vitro and allergic reaction of mice and rats. The anti-fertility effect of PLGA-LNG microphere composite on the same animals also has been tested. The studies demonstrated that PLGA had fine biological compatibility without side effects such as toxicity,irritations on conjunctiva and muscle,pyrogenicity,haemolysis in vitro and allergic reaction. The contraception period of mice lasted 11. 5, 34. 6 and 81. 2 days respectively after the subcutaneous (s. c. ) injection of PLGA-LNG microphere which contains LNG 5,10 and 20 mg/kg separately. The intramuscular(i. m. ) injection of PLGA-LNG microphere containing LNG 13. 6,19. 8 and 30 mg/kg respectively could keep the estrus of rats supressed for 76. 3, 104. 1 and 131. 8 days. The injections of PLGA-LNG microphere through s. c. or i. m. showed rnore significant effects than the same dosages of PLGA-LNG micro-crystal. These results suggest that PLGA-LNG microphere injection has long-term anti-fertility effect.

关 键 词：丙交酯乙交酯共聚物 毒性 LNG 微球 抗生育作用 

分 类 号：R318.08[医药卫生—生物医学工程]