欧盟鱼病水平测试样品中DNA病毒检测  

Detection of DNA Virus in the Inter-laboratory Proficiency Test

作　　者：徐晔[1] 段宏安[1] 周毅[1] 丁超[1] 

机构地区：[1]连云港出入境检验检疫局,江苏连云港222042

出　　处：《安徽农业科学》2012年第34期16624-16626,共3页Journal of Anhui Agricultural Sciences

基　　金：质检公益项目(201110037);国家质检总局科技计划项目(2010IK014);江苏检验检疫局科研项目(2007KJ01)

摘　　要：[目的]通过参加欧盟鱼病水平测试,评价试验室检测能力,加强对检测方法的掌握。[方法]按照水平测试说明的方法进行病毒分离鉴定,测试的10个样品在病毒分离后用PCR方法检测DNA病毒。[结果]PTⅡ检测为流行性造血器官坏死病毒(Epizootic haemato-poietic necrosis virus,EHNV),PTⅢ为欧洲鮰病毒(European catfish virus,ECV),PTⅧ和PTⅩ为锦鲤疱疹病毒(Koi herpesvirus disease vi-rus,KHDV),PTⅨ为流行性溃疡综合征(Epizootic ulcerative syndrome,EUS)。[结论]该实验室检出了2011年欧盟鱼病水平测试样品中的4种DNA病毒,与最后公布的测试结果完全一致。[ Objective ] By participating the European Union inter-laboratory proficiency test for fish diseases, the detectability of the laborato- ry was evaluated and the master of the detection method was improved. [ Method] Ten proficiency test samples were detected by virus isolation and identification according to the instruction of the proficiency test. [Result] The PTⅡ was epizootic haematopoietie necrosis virus（EHNV）, the PTⅢ was European catfish virus（ ECV）, the PTⅧ and PTX were both koi herpesvirus disease virus（ KHDV）, the PTⅨ was epizootic ul- cerative syndrome（EUS）. [ Conclusion] Four types of DNA virus were detected positive from the proficiency test samples, which was consist- ent with the final results.

关 键 词：鱼病水平测试 DNA病毒 欧盟 

分 类 号：S941[农业科学—水产养殖]