新鲜脐血及其不同温度保存下凝血因子活性的动态测定  

Activity changes of coagulation factors in umbilical cord plasma stored at different temperature for different duration

作　　者：王芝琪[1] 周志健[1] 杨英姿[1] 董玫 李璐璐[1] 苏雁华[1] 崔淑杰[1] 孟德润[1] 

机构地区：[1]哈尔滨医科大学附属第二医院血液内科,黑龙江哈尔滨150086

出　　处：《哈尔滨医科大学学报》2000年第3期177-178,共2页Journal of Harbin Medical University

摘　　要：目的了解输注脐血凝血因子的活性。方法采集 １０份新鲜脐血，对保存前及其在４℃、－２０℃保存２４ｈ、４８ｈ后各凝血因子活性进行了动态检测。结果①脐血处于相对低凝状态；②脐血在４℃、－２０℃保存２４ｈ、４８ｈ后其纤维蛋白原、因子Ⅴ、Ⅷ都呈现不同程度的衰减，且４℃和－２０℃保存效果无显著差异，而其余因子则储存稳定。结论新鲜脐血中各凝血因子含量较低，且随保存时间的延长活性下降。Objective To explore the activities of coagulation factors in umbilical cord plasma. Methods An ACL-200(Automated Coagulation Laboratory) was used to detected the activities of coagulation factors in 10 fresh umbilical cord plasma, and their changes after the plasma was stored at 4℃, -20℃ for 24 hours and 48 hours. Results ①Umbilical cord plasma was in a relatively hypocoagulation status; ②Fibrinogen, some factors declined notably after the plasma was stored at 4℃ and -20℃ and there was no obvious difference between the two storage conditions. Conclusion The amount of coagulation factors in fresh umbilical cord plasma is low and the activity is declined with the duration of storage postponing.

关 键 词：脐带血血浆 凝血因子 活性 温度 保存 

分 类 号：R457[医药卫生—治疗学] R617[医药卫生—临床医学]