Influence and mechanism of N-(3-oxooxtanoyl)-L-homoserine lactone(C_8-oxo-HSL) on biofilm behaviors at early stage  

作　　者：Siqing Xia Lijie Zhou Zhiqiang Zhang Jixiang Li 

机构地区：[1]State Key Laboratory of Pollution Control and Resource Reuse,Tongji University,Shanghai 200092,China [2]Sustainable Technology Research Center,Shanghai Advanced Research Institute,Chinese Academy of Sciences,Shanghai 201210,China

出　　处：《Journal of Environmental Sciences》2012年第12期2035-2040,共6页环境科学学报（英文版）

基　　金：supported by the National High Technology Research and Development Program(863)of China(No.2009AA062902)

摘　　要：N-acyl-homoserines quenching, enzymatic quenching of bacterial quorum sensing, has recently applied to mitigate biofilm in membrane bioreactor. However, the effect of AHLs on the behavior of biofilm formation is still sparse. In this study, Pseudomonas aeruginosa biofilm was formed on ultra-filtration membrane under a series of N-（3-0xooxtanoyl）-L-homoserine lactone （C8-0xo-HSL） concentrations. Diffusing Cs-oxo-HSL increased the growth rate of cells on biofilm where the concentration of Cs-oxo-HSL was over 10-7 g/L. The C8-0xo-HSL gradient had no observable influence on cell density and extracellular polymeric substances of biofilm with over 10-7 g/L Cs-oxo-HSL. Surprisingly, 10-11-10-8 g/L of Cs-Oxo-HSL had no effect on cell growth in liquid culture. The cell analysis demonstrated that the quorum sensing system might enhance the growth of neighboring cells in contact with surfaces into biofilm and may influence the structure and organization of biofilm.N-acyl-homoserines quenching, enzymatic quenching of bacterial quorum sensing, has recently applied to mitigate biofilm in membrane bioreactor. However, the effect of AHLs on the behavior of biofilm formation is still sparse. In this study, Pseudomonas aeruginosa biofilm was formed on ultra-filtration membrane under a series of N-（3-0xooxtanoyl）-L-homoserine lactone （C8-0xo-HSL） concentrations. Diffusing Cs-oxo-HSL increased the growth rate of cells on biofilm where the concentration of Cs-oxo-HSL was over 10-7 g/L. The C8-0xo-HSL gradient had no observable influence on cell density and extracellular polymeric substances of biofilm with over 10-7 g/L Cs-oxo-HSL. Surprisingly, 10-11-10-8 g/L of Cs-Oxo-HSL had no effect on cell growth in liquid culture. The cell analysis demonstrated that the quorum sensing system might enhance the growth of neighboring cells in contact with surfaces into biofilm and may influence the structure and organization of biofilm.

关 键 词：quorum sensing C8-oxo-HSL biofilm formation ultra-filtration membrane cell density extracellular polymericsubstances 

分 类 号：Q73[生物学—分子生物学] Q517