Distribution,Frequency and Variation of Stripe Rust Resistance Loci Yr10,Lr34/Yr18 and Yr36 in Chinese Wheat Cultivars  

作　　者：Cuiling Yuan Hui Jiang Honggang Wang Kun Li Heng Tang Xianbin Li Daolin Fu 

机构地区：[1]State Key Laboratory of Crop Biology,Shandong Key Laboratory of Crop Biology,Shandong Agricultural University,61 Daizong Street,Tai'an,Shandong 271018,China

出　　处：《Journal of Genetics and Genomics》2012年第11期587-592,共6页遗传学报（英文版）

基　　金：supported by the China Research and Development Initiative on Genetically Modified Plants(Grant No.2009ZX08009- 053B);the National Basic Research Program of China(973 Program,Grant No.2011CB 100700);the National Natural Science Foundation of China(Grant No.30871323)

摘　　要：Wheat stripe rust is a devastating disease in many regions of the world. In wheat, 49 resistance genes for stripe rust have been officially documented, but only three genes are cloned, including the race-specific resistance YrlO candidate gene （YrlOca） and slow-rusting genes Lr34/Yr18 （hereafter designated as Yrl8） and Fr36. In this study, we developed gene-specific markers for these genes and used them to screen a collection of 659 wheat accessions, including 485 Chinese cultivars. Thirteen percent and eleven percent of the tested Chinese cultivars were positive for the markers for YrlOca and Yr18nn （the resistant haplotype of YrlS）, respectively, but none were positive for the Yr36 marker. Since there is a limited use of the YrlO gene in Chinese wheat, the relatively high frequency of wheat varieties with the YrlOcG marker suggests that the identity of the YrlO gene is unknown. With regards to the Yr18 gene, 29% of the tested cultivars that are used in the Middle and Lower Yangtze Valleys＇ winter wheat zone were positive for Yrl8RH markers. A non-functional allele of Yrl8Rn was identified in ＇Mingxian 169＇, a commonly used susceptible check for studying stripe rust. The data presented here will provide useful information for marker-assisted selection for wheat stripe rust resistance.Wheat stripe rust is a devastating disease in many regions of the world. In wheat, 49 resistance genes for stripe rust have been officially documented, but only three genes are cloned, including the race-specific resistance YrlO candidate gene （YrlOca） and slow-rusting genes Lr34/Yr18 （hereafter designated as Yrl8） and Fr36. In this study, we developed gene-specific markers for these genes and used them to screen a collection of 659 wheat accessions, including 485 Chinese cultivars. Thirteen percent and eleven percent of the tested Chinese cultivars were positive for the markers for YrlOca and Yr18nn （the resistant haplotype of YrlS）, respectively, but none were positive for the Yr36 marker. Since there is a limited use of the YrlO gene in Chinese wheat, the relatively high frequency of wheat varieties with the YrlOcG marker suggests that the identity of the YrlO gene is unknown. With regards to the Yr18 gene, 29% of the tested cultivars that are used in the Middle and Lower Yangtze Valleys＇ winter wheat zone were positive for Yrl8RH markers. A non-functional allele of Yrl8Rn was identified in ＇Mingxian 169＇, a commonly used susceptible check for studying stripe rust. The data presented here will provide useful information for marker-assisted selection for wheat stripe rust resistance.

关 键 词：Puccinia striiformis Stripe rust Triticum aestivum 

分 类 号：S512.1[农业科学—作物学] S888.3