RGMa在创伤后精神应激障碍大鼠内侧前额皮质的表达变化  

Expression of Repulsive Guidance Molecule in the Medial Prefrontal Cortex of Post-traumatic Stress Disorder Rats

作　　者：温莉莉[1] 韩芳[1] 石玉秀[1] 

机构地区：[1]中国医科大学基础医学院组织学与胚胎学教研室,沈阳110001

出　　处：《中国医科大学学报》2012年第12期1065-1067,1072,共4页Journal of China Medical University

基　　金：国家自然科学基金资助项目(31140060)

摘　　要：目的探讨创伤后精神应激障碍(PTSD)大鼠内侧前额皮质排斥性导向分子(RGMa)的表达变化。方法将45只健康成年雄性Wistar大鼠随机分为对照组(15只)、SPS7 d组(15只)和SPS14 d组(15只),采用无连续单一应激(SPS)方法制备PTSD模型。用免疫组织化学和免疫印迹法检测内侧前额皮质RGMa的表达变化。结果免疫组织化学分析显示,对照组、SPS7d组和SPS14d组RGMa阳性信号OD值分别是(0.44±0.002)、(0.63±0.015)和(0.51±0.009),差异有统计学意义(P<0.05);免疫印迹分析显示,对照组、SPS7d组和SPS14d组RGMa相对表达灰度值分别为(0.556 817±0.009 85)、(0.778 967±0.028 325)和(0.659 633±0.030 979),差异有统计学意义(P<0.05)。结论 PTSD大鼠内侧前额皮质RGMa表达增强,SPS7d达到高峰,RGMa的表达增加可能参与了PTSD的病理生理过程。Objective To observe the expression of repulsive guidance molecule A （RGMa） in the medial prefrontal cortex （mPFC） of rats with posttraumatic stress disorder（ PTSD ）. Methods Male Wistar rats were randomly divided into control group,SPS 7 d group and SPS 14 d group. The rats of SiX5 7 d and SPS 14 d groups were exposed to the single-prolonged stress （SPS） to set up the rat PTSD models. The expression of RGMa was detected using immunohistochemistry, Western blotting, image analysis and statistical analysis. Result The result of immunohistochemistry analysis showed that the expression level of RGMa gradually increased, peaking at SPS 7 d, and then gradually de- creased at SPS 14 d but still higher than in the control group （P 〈 0.05） . The Western blot result consistent with inmmnohistochemistry analysis. Conclusion The expression of RGMa was significantly increased in mPFC of PTSD model rats. The RGMa signal transduction pathway in mPFC might play an important role in the pathogenesis of PTSD.

关 键 词：创伤后应激障碍 内侧前额皮质 RGMa 免疫印迹 免疫组织化学 

分 类 号：R329.4[医药卫生—人体解剖和组织胚胎学]