Survivin基因转染对骨髓间充质干细胞体外抗凋亡能力的影响  

Effects of Survivin on Anti-apoptotic Capacity in Bone Marrow Mesenchymal Stem Cells

作　　者：范红杰[1] 王倩[1] 冯娟[1] 于维东[2] 张强[2] 

机构地区：[1]中国医科大学附属盛京医院神经内科,沈阳110004 [2]中国医科大学附属第一医院干诊科,沈阳110001

出　　处：《中国医科大学学报》2012年第12期1080-1082,1087,共4页Journal of China Medical University

基　　金：国家自然科学基金资助项目(81070254);辽宁省教育厅高校科研计划(05L485)

摘　　要：目的观察存活素(Survivin)基因转染对大鼠骨髓间充质干细胞的体外抗凋亡作用。方法 PCR扩增Survivin全长编码基因,通过脂质体介导将Survivin基因转染至骨髓间充质干细胞,RT-PCR检测转染后Survivin mRNA的表达,Annexin V/PI双标法检测去血清诱导后细胞凋亡比例。结果 RT-PCR显示转染组有效表达Survivin mRNA,转染组去血清培养后的早期凋亡率较未转染组降低,差异有统计学意义(P<0.05)。结论 Survivin基因转染对骨髓间充质干细胞有保护性作用,可增强其抗凋亡能力,延长其存活时间。Objective To observe the in vitro biological characteristics and anti-apoptotic effects of rat bone mesenchymal stem ceils （BMSCs） transfected with Survivin. Methods The Survivin coding sequence was amplified by polymerase chain reaction （PCR） method and sub- cloned into liposome. The plasmid was transfected into BMSCs, and RT-PCR method was used to detect the Sunvin expression. Annexin V- PI double staining method was used to detect cell apoptosis proportion. Results RT-PCR results confirmed that the transfected group effec- tively express Surcivin mRNA compared with controls. Early apoptosis rate of the transfected group was significantly lower than the control group （P 〈 0.05 ）. Conclusion Survivin gene transfection can enhance the anti-apoptotic ability of the BMSCs so as to extend their sur- vival time.

关 键 词：骨髓间充质干细胞 SURVIVIN基因 转染 凋亡 

分 类 号：R34[医药卫生—基础医学]