响应面法优化豆粕发酵产共轭亚油酸条件  

Optimization of Soybean Meal Fermentation Conditions for the Production of Conjugated Linoleic Acid(CLA) by Response Surface Methodology

作　　者：赵蓉蓉[1] 桑卫国[1] 

机构地区：[1]宁波大学海洋学院,宁波315211

出　　处：《中国粮油学报》2012年第12期62-68,共7页Journal of the Chinese Cereals and Oils Association

基　　金：宁波市科技局项目(2010C10059);宁波市自然科学基金(2010A610007)

摘　　要：为寻求以豆粕为主要基质产共轭亚油酸最佳发酵条件,采用响应面法优化其发酵条件。在单因素试验基础上,选择不同接种量、油脂添加量、发酵时间为自变量,共轭亚油酸产量为响应值,利用Box-Benhnken中心组合方法进行3因素3水平的试验设计,并做响应面分析,建立回归模型。结果表明,回归方程拟合性好,发酵最优条件为:接种量3.2%,油脂添加量1.93 g·(50 g豆粕)-1,初始pH 6.0,在37℃发酵80 h。在此条件下,发酵豆粕中共轭亚油酸产量可达700.32μg/g。这为生物发酵法生产共轭亚油酸提供研究基础。This experiment was studied on the optimization fermentation condition for the production of CLA with the soybean meal as the matrix. Response surface methodology was used to optimize the fermentation conditions. On the basis of single -factor test,the mathematical regression model was established by selecting the response value （ yield of CLA） and independent variables （ inoculums size, oil addition, fermentation time ） through Box - Benhnken center combined method for the testing design of three - factor and three - level and doing response surface analysis. According to analysis, regression equation fitting is good and the optimal fermentation conditions for inoculums size 3.2% （V/V） ,oil addition 1.93 g （50 gsoybean meal） -1 ,initial pH 6.0 and at 37℃ for fermentation time 80 h re- spectively. Under this condition, the yield of CLA could reach to 700.32 μg/g. It provided research foundation for bio- logical fermentation process producing . conjugated linoleic acid.

关 键 词：豆粕 共轭亚油酸响 应面分析 

分 类 号：TS225.1[轻工技术与工程—粮食、油脂及植物蛋白工程] Q939.117[轻工技术与工程—食品科学与工程]