解毒活血法对UUO大鼠IL-1β和TNF-α的影响  

Effects of IL-1 β and TNF-α in the UUO rat by the detoxicating and activating blood method

作　　者：魏民[1] 杨京京[1] 王建琴 王霞[1] 王香婷[1] 许庆友[3] 

机构地区：[1]河北医科大学中医学院,石家庄050091 [2]河北省蔚县卫生局,河北蔚县075700 [3]河北医科大学中西医结合学院,石家庄050091

出　　处：《中国中医基础医学杂志》2012年第12期1337-1339,共3页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：河北省科技支撑计划项目(10276105-D);河北省中医药管理局科研计划项目共同资助(2010005)

摘　　要：目的:通过观察解毒活血法对大鼠肾组织白细胞介素-1β(interleukin-1β,IL-1β)、肿瘤坏死因子-α(tumour necrosis factor-α,TNF-α)的影响,探讨其对UUO大鼠的保护作用及可能机制。方法:采用结扎左侧输尿管的方法复制UUO大鼠模型。治疗组大鼠分别灌服缬沙坦26mg/kg.d,解毒活血中药12g/kg.d,24g/kg.d,共14d。放射免疫法检测IL-1β,免疫组化检测肾组织中TNF-α的蛋白表达。结果:模型组大鼠IL-1β和TNF-α的表达明显增高,各治疗组表达较模型组明显减弱。结论:解毒活血方药可降低肾组织中IL-1β和TNF-α的高表达,通过抑制炎症反应减缓肾小管间质纤维化的进程。Aim： To observe the effects of Jiedu Huoxue therapy on the expression of interleukin-1β3 （IL-1β） and tumour necrosis factor-or （TNF-α） in UUO rats, and to explore the protective effect and possible mechanism of Jiedu Huoxue therapy in UUO rats. Methods：Rats were made into UUO model by ligaturing the left ureter and treated respectively with Valsartan 26mg/kg.d, Jiedu Huoxue decoction 12g/kg. d and 24g/kg.d for fourteen days. Take the left kidney to detect IL-1β by radioimmunoassay. The protein expressions of TNF-α in rats kidney were detected by immunohistochemical. Results：The expression of IL-1β and TNF-α were increased significantly in the model group. After treatment, the expression of IL-1β and TNF-α were significantly down-regulated in the treatment groups. Conclusions Jiedu Huoxue decoction is able to reduce the high expressions of IL-1β and TNF-α, and to retard the progress of renal tubular interstitial fibrosis by inhibiting inflammatory reaction.

关 键 词：肾小管间质纤维化 单侧输尿管梗阻 解毒活血法 白细胞介素-1Β 肿瘤坏死因子-α 

分 类 号：R285.5[医药卫生—中药学]