补中益气法对甲减大鼠心肌α-MHC和β-MHCmRNA表达的影响  

The influence of Bu Zhong Yi Qi method on themyocardial α-MHC and β-MHCmRNA expression in hypothyroid rats

作　　者：高天舒[1] 尹慧丝[2] 

机构地区：[1]辽宁中医药大学附属医院内分泌科,沈阳110032 [2]辽宁大学医院,沈阳110031

出　　处：《中国中医基础医学杂志》2012年第12期1340-1343,1352,共5页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：辽宁省教育厅资助项目(2009A492);教育部博士点基金项目(20092133110004);辽宁省教育厅优秀人才计划(LR201026)

摘　　要：目的:研究补中益气法对甲状腺功能减退(甲减)大鼠心肌肌球蛋白重链α和β(myosin heavy chain alpha and beta,α-MHC和β-MHC)mRNA表达的影响。方法:将甲减Wistar大鼠随机分为模型对照组、L-T4组、补中益气汤组。实时定量RT-PCR法测心肌α-MHC和β-MHC mRNA。结果:给处理因素8周,L-T4组、补中益气汤组的α-MHCmRNA的表达分别为正常组的1.77倍、2.32倍,补中益气汤组较L-T4组上升明显(P<0.05);L-T4组、补中益气汤组β-MHCmRNA的表达分别为正常组的2.61倍、1.17倍,补中益气汤组下调明显(P<0.05)。结论:补中益气法在甲减心肌损伤的修复中起重要作用,其机制与其提高心肌α-MHC mRNA的表达、降低β-MHCmRNA的表达有关。Objective： To observe the effect of Buzhong Yiqi method on the expression of hypothyroid rats myocardial myosin heavy chain alpha and beta mRNAs. Methods： Hypothyroid Wistar rats were randomly divided into model group, L- T4 group, Buzhongyiqi decoction group. The expression of mRNA of myocardial myosin heavy chain alpha and beta were measured with Real-time RT-PCR.. Results： 8 weeks later, The expression of α-MHC mRNA of L-T4 group and Buzhongyiqi decoction group was 1.77 times and 2.32 times respectively higher than normal group, Buzhongyiqi decoction group increased obviously（ P 〈 0. 05 ） ;The expression of β-MHC mRNA of L-T4 group and Buzhongyiqi decoction group was 2. 61 times and 1.17 times respectively higher than normal group, compared with model group, both are reduced, but all did not drop to normal levels, Buzhongyiqi decoction group decreased obviously （P 〈 0.05 ）. Conclusion： Buzhongyiqi method play an important role on repairing hypothyroid myocardial injury, which is related to improving the alpha MHCmRNA expression and reducing the beta MHCmRNA expression.

关 键 词：补中益气法 甲减 α-MHC Β-MHC 

分 类 号：R285.5[医药卫生—中药学]