中医骨折三期治疗对家兔骨折愈合中VEGF与BMP-2表达影响的实验研究  

Experimental study of the VEGF and BMP-2 expression on fracture healing of rabbits by the three stage treatment of traditional Chinese medicine fractures

作　　者：毕锴[1] 温建民[1] 孙永生[1] 桑志成[1] 徐颖鹏[2] 孙卫东 高国庆[3] 胡海威[1] 董颖 

机构地区：[1]中国中医科学院望京医院,北京100102 [2]中国中医科学院广安门医院,北京100053 [3]怀柔区卫生局,北京110000 [4]怀柔区社区康复中心,北京101400

出　　处：《中国中医基础医学杂志》2012年第12期1344-1347,共4页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家自然科学基金资助项目-中医骨折不同分期治疗对骨折愈合的作用及其机制的实验研究(30973762)

摘　　要：目的:观察中医骨折三期治疗在骨折愈合中VEGF与BMP-2表达的影响,探讨其合理性。方法:家兔建立骨折模型(n=132),随机抽6只为对准组,其余分为三期组、一期组、模型对照组(n=42)。每组分为3、6、9、14、28、42、56d(n=6)。并进行取材,组织学及免疫组化法检测分析。结果:各组术后骨折端两因子表达阳性细胞平均光密度(MOD)值:三期组>一期组>模型组(P<0.05)。结论:中药治疗能显著促进骨折愈合中VEGF与BMP-2表达,三期较强,骨折分期治疗非常必要。Objective：To observe the effects of the three period treatment theory of bone fracture in TISM （TraditIonal Chinese Medicine）on VEGF and BMP-2 expression of rabbits in the issues of fracture healing, and to explore the rationality of that method. Methods： Rabbits（ n = 132） were make bone fracture models. Six rabbits randomly take into nomal group, and others were randomly divided into three groups, including three-phase treatment group （TTG） （n = 42 ）, one-phase treatment group （OTG） （ n = 42）, and model control group（MCG） （ n = 42）. Every group have 3,6,9,14,28,42and56 day point（n = 6） , The left radiuses were taken out as the research object. Immunobistochemistry stain was performed to examinate the VEGF and BMP-2 expression in the fracture bone. Results： Group mean MOD（ mean optical density）in positive cell of VEGF and BMP-2 expression in every group at deferent time points and those of more time points after operation：TTG 〉 OTG 〉 MCG （ P 〈 0.05 ）. Conclusion： Treating fracture in stages has more predominant effect on the expression of VEGF and BMP-2 than that of treating fracture with single prescription. The period treatment for fracture is very necessary

关 键 词：骨折 血管内生长因子类 骨形态发生蛋白类 免疫组化 家兔 

分 类 号：R274.1[医药卫生—中医骨伤科学]