一氧化氮信息传递系统对大鼠呼吸道纤毛运动的影响  

Effect of NO signal system on rat airway ciliary motility

作　　者：展新华[1] 张锦[2] 盛卓人 

机构地区：[1]同济医科大学协和医院麻醉科,武汉市430022 [2]中国医科大学附属二院麻醉科

出　　处：《中华麻醉学杂志》2000年第3期159-161,共3页Chinese Journal of Anesthesiology

摘　　要：目的　了解一氧化氮 (NO)信息传递系统对呼吸道上皮细胞纤毛运动频率 (CBF)的影响。方法　选用雄性SD大鼠 9只 ,异氟醚麻醉后以无菌技术迅速取其气管 ,并将气管粘膜切成1mm2大小的组织块 ,在DMEM中进行组织培养。将组织块分成 5组 :L 精氨酸 (L Arg)组 ;1 Hy droxy 2 oxo 3 (N ethyl 2 aminoethyl) 3 ethyl 1 triazene (NOC 12 ,NO载体 )组 ;8 Br cGMP组 ;D Arg组及磷酸盐缓冲液 (PBS)组。以相差显微镜及成像分析技术测定CBF ,对纤毛运动进行定量分析。结果　 (1)给L Arg后CBF由 (7 43+0 75 )Hz增至 (8 5 9+0 93)Hz(P <0 0 1) ;给NOC 12后CBF由 (7 6 6 +0 6 8)Hz增至 (8 6 2 +0 91)Hz(P <0 0 5 ) ;给 8 Br cGMP后CBF由 (7 11+0 6 6 )Hz增至(8 10 +0 83)Hz(P <0 0 5 ) ;(2 )PBS及D Arg对CBF无影响。 结论　NO信息传递系统能使大鼠呼吸道上皮细胞CBF加快。Objective To understand the effects of NO signal system on the ciliary beating frequency (CBF) of airway epithelial cellMethods Nine normal male Sprague-Dawley rats were anesthetized with isoflurane Their tracheas were rapidly removed using aseptic technique The mucosa of trachea were cut into 1mm2 explants and cultured in DMEM The explants were divided into 5 groups as bellow: L-Arg group, 1-Hydroxy-2-oxo-3-(N-ethyl-2-aminoethyl)-3-ethyl-1-triazene (NOC-12) group, D-Arg group, 8-Br-cGMP group, and phosphate buffered saline (PBS)group Actively beating ciliated cells were observed, and their motion was quantified by measuring CBF using phase-contrast microscopy and videotape analysis Results L-Arg increased CBF from (7 43±0 75)Hz to(8 59±0 93)Hz (P<0 01),NOC-12 increased CBF from (766±068) Hz to (862±0 91)Hz (P<005), and 8-Br-cGMP increased CBF from (711±066)Hz to (810±0 83)Hz (P<005)PBS and D-Arg did not change CBF significantlyConclusions NO signal pathway increases the CBF of airway epithelial cell

关 键 词：一氧化氮 一氧化氮合酶 呼吸道 纤毛运动 

分 类 号：R332[医药卫生—人体生理学]