中枢NO—cGMP系统对大鼠吗啡戒断症状的影响  

Effect of Central NO-cGMP System on Morphine Withdrawal Symptoms in Morphine Dependent Rats

作　　者：杨彦玲 刘秀平 邱学才[1] 李静[1] 刘晓红[1] 徐丽[1] 卢景芬[1] 

机构地区：[1]北京医科大学生理学系

出　　处：《中国心理卫生杂志》2000年第2期110-113,共4页Chinese Mental Health Journal

摘　　要：目的 :观察中枢NO -cGMP系统对大鼠吗啡戒断症状的影响。方法 :用皮下递增注射吗啡法建立吗啡依赖模型 ,腹腔注射纳洛酮催瘾。侧脑室注射 (i c v )不同药物 ,观察对大鼠叩齿、扭体、湿狗样抖动、舔阴茎、逃逸、体重丢失等吗啡戒断症状的影响。结果 :微量侧脑室注射 (i c v )NO的供体硝普钠(SNP)、NO的前体L -精氨酸 (L -Arg)、环磷酸鸟苷 (cGMP)以增加脑内NO的释放 ,激活鸟苷酸环化酶(GC) ,提高脑内cGMP水平 ,结果使吗啡戒断症状加重 ;相反 ,微量注射NO合酶 (NOS)抑制剂NG-硝基 -L精氨酸 (LNNA)及鸟苷酸环化酶 (GC)抑制剂甲基兰 (MB) ,使脑内NO生成减少 ,降低NO对GC的激活作用 ,脑内cGMP生成减少 ,则可使大鼠吗啡戒断症状减轻。结论 :NO -cGMP系统在产生吗啡戒断反应中具有重要作用。Objective: To observe the effect of central NO-cGMP system on morphine withdrawal symptoms in morphine dependent rats Method: A physical morphine dependent model in rats was established by subcutaneous injection of morphine in gradually increasing doses (from 5mg/kg to 50mg/kg in five days) After intraperitoneal injection of naloxone (0 5mg/kg), withdrawal symptoms including teeth chattering, writhing, wet shakes, penile licking, escape attempts and weight loss were scored to assess the effects of various drugs by intracerebroventricular microinjection(icv) on them Results: Morphine wiehdrawal symptoms were aggravated due to the release of NO and the activation of guanosine cyclase (GC) by icv of the donor of NO (sodium nitroprusside), the precursor of NO (L-Arg) and cGMP In contrast, those symptoms were relieved by microinjection of the inhibitor of NO synthetase (NG-nitro-L-Arginine, NNLA) and the inhibitor of GC (methylene blue, MB) Conclusion: NO-cGMP system plays an important role in the development of morphine withdrawal symptoms in rats

关 键 词：NO-cGMP系统 硝普钠 吗啡戒断症状 

分 类 号：R595.5[医药卫生—内科学] R163[医药卫生—临床医学]