前列地尔对脓毒症大鼠血清iNOS和TNF-α表达的影响  

The Effects of Alprostadil on Serum iNOS and TNF-α of Septic Rats

作　　者：杨建平[1] 翁金森[1] 林静萍[1] 林建东[2] 

机构地区：[1]福建省肿瘤医院ICU,福建福州350014 [2]福建医科大学附属第一医院重症医学科,福建福州350005

出　　处：《中国医药指南》2012年第33期62-63,共2页Guide of China Medicine

摘　　要：目的研究前列地尔对脓毒症大鼠血清中iNOS和TNF-α表达的影响。方法 45只wistar大鼠随机等分为前列地尔组、脓毒症组和对照组。脓毒症模型则以盲肠结扎穿孔(CLP)来构建,对照组行除CLP外的相同手术。前列地尔组于制模前1h尾静脉注射注射前列地尔10μg/kg,对照组和脓毒症组则分别静脉注射平衡液5mL/kg,术后每隔8h同样给药一次。术后12、24h尾静脉采集血标本检测血清中iNOS和TNF-α水平。结果前列地尔组大鼠血清中iNOS和TNF-α表达水平低于脓毒症组且有显著意义。结论前列地尔可能通过调节iNOS和TNF-α表达量从而起到对脓毒症脏器的保护作用。Objective To study the effects of alprostadil on serum iNOS and TNF-α of septic rats.Methods 45 wistar rats were randomly divided into the alprostadil group,sepsis group and control group.Septic model was induced by cecal ligation and puncture（CLP）.The rats of control group were made by the same surgery except CLP.Rats of alprostadil group were injected alprostadil 10μg/kg 1h before surgery.The rats of control group and sepsis group were injected intravenously with balanced salt solution 5mL/kg.And then rats of three groups were made by the same administration every eight hours.Blood samples were collected through tail vein 12 and 24 hours after surgery.And then the serum of iNOS and TNF-α levels were detected.Results The iNOS and TNF-α levels of alprostadil group were significantly lower than those in the sepsis group.Conclusion Alprostadil may through regulating the expression of iNOS and TNF-α expression levels to play a protective effect on organs of septic rats.

关 键 词：前列地尔 脓毒症 脏器保护 

分 类 号：R631[医药卫生—外科学]