富勒烯的化学修饰技术及其修饰产物在疾病预报中的应用  

Chemically Modified Technique of Fullerene and its Application in Diseases Prediction

作　　者：李园园 李志明[2] 郑志勇[2] 

机构地区：[1]漳州科技职业学院,福建漳州363202 [2]漳州职业技术学院,福建漳州363000

出　　处：《漳州职业技术学院学报》2012年第4期7-12,共6页Journal of Zhangzhou Institute of Technology

基　　金：福建省教育厅科技项目资助(JB11308)

摘　　要：开发了N,N-二乙基苯胺(DEA)-富勒醇(F)-异硫氰酸荧光素(FITC)磷光标记试剂(DEA-F-FITC)及建立DEA-F-FITC标记伴刀豆凝集素(Con A)亲和吸附(AA)固体基质室温燐光法(AA-SS-RTP)测定AFP-V新方法。该方法的灵敏度高(Pg/mL)为0.13(F)和0.090(FITC),用于测定人血清样品中AFP-V的含量和人体疾病预报,结果都与酶联免疫法的相吻合。The phosphorescent labeling reagent N, N-dimethylaniline-fullerol-fluorescein isothiocyanate （DEA-F-FITC） has been developed. A new affinity adsorption-solid substrate-room temperature phosphorimetry （AA-SS-RTP） for the determination of alpha-fetoprotein variant （AFP-V） had been established, based on DEA-F-FITC as a labeling reagent of concanavalin agglutinin （Con A）. This method has high sensitivity （The detection limits （Pg/mL） are 0.13 for F and 0.090 for FITC, respectively）, which has been applied to the determination of trace AFP-V in human serum and the forecast of human diseases, and the results agreed well with those obtained by enzyme-linked immunoassay.

关 键 词：富勒烯 化学修饰技术 应用 

分 类 号：O613.71[理学—无机化学]