穿琥宁注射液类过敏反应的实验研究  

The Experimental Study on Anaphylactoid Reactions of Potassium Dehydroandrograpolide Succinate Injection

作　　者：李春英[1] 梁爱华[1] 赵雍[1] 易艳[1] 王云庭[1,2] 李桂琴[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]首都医科大学中医药学院,100069

出　　处：《中国执业药师》2012年第12期45-48,共4页China Licensed Pharmacist

基　　金：国家自然科学基金(90709043);国家科技支撑计划项目(2006BAI14B05);国家科技重大专项(2009ZX09301-005;2009ZX09502-001)

摘　　要：目的:研究小鼠和大鼠对穿琥宁注射液的类过敏反应,为其临床用药提供参考。方法:①小鼠全身类过敏试验:ICR小鼠随机分组,分别一次性静脉注射生理盐水、Compound 48/80溶液或不同浓度穿琥宁注射液,以上受试物中均含0.4%伊文思蓝。给药后30min记录各组动物耳廓蓝染阳性率,进行耳廓蓝染程度评分,并定量测定耳廓伊文思蓝渗出量。②大鼠皮肤类过敏试验:将大鼠背部和脊柱两侧的毛剃去,经尾静脉注射含0.6%伊文思蓝的生理盐水溶液2mL/kg,10min后,在麻醉条件下沿脊柱两侧皮内注射受试药,每个点注射50μL。每只鼠均平行设有阴性对照点(生理盐水50μL)和阳性对照点(Compound 48/80500μg)。20min后,掀开皮肤观察皮肤内蓝斑形成情况,测量蓝斑大小。结果:小鼠静脉注射穿琥宁注射液50、100、200mg/kg(分别相当于临床剂量的1/2、等倍和2倍剂量)均可引起小鼠类过敏反应,表现为耳廓血管通透性增高,耳廓不同程度蓝染;大鼠皮内注射穿琥宁注射液50、100μg,可观察到注射局部明显水肿,形成较大的蓝斑(与阴性对照组比较P<0.05)。结论:穿琥宁注射液对小鼠和大鼠均可引起显著的类过敏反应,提示在临床治疗中具有潜在的类过敏反应的可能。Objective： To investigate the anaphylactoid reactions of potassium dehydroandrograpolide succinate injection （PDSI） in mice and rats so as to provide a scientific guidance for its clinical therapy. Methods： @Anaphylactoid reaction test in mice： ICR mice were randomly assigned to receive intravenously physiological saline, compound 48/80 solution or different concentrations of PDSI respectively, containing 0.4% Evans blue （EB） . Thirty minutes later, the auricle aizen positive rate of each animal was recorded, the auricle aizen degree score and the quantitative determination of auricle EB seepage quantity were made. （2）Cutaneous anaphylaxis test in rats： rats were given physiological saline solution containing 0.6% EB by tail vein intravenous injection. Ten minutes later, 50 μL solutions of PDSI in different concentrations were given to anesthetized rats by intradermal injection on both sides of spine. Each rat is parallel with negative control point （physiological saline 50μL） and positive control point （compound 48/80 500 μg）. Twenty minutes later, blue plaque in skin was measured and the absorbance of EB extraction of skin was quantitative determined. Results： The study of anaphylactoid reaction test in mice showed that all three doses of PDSI （50, 100 and 200 mg/kg, equivalent respectively to 1/2, 1 and 2 times of clinical doses） induced anaphylactoid reactions, as the vascular permeability increased and ear blue stained at different extent. The study of cutaneous anaphylaxis test in rats showed that PDSI at a dose of 50 and 100 mg/kg can induce edema at the injection site and form a larger locus blue plaque （vs. the negative control group, P〈 0.05 ）. Conclusion： It was proved that anaphylactoid reactions can be induced evidently by PDSI both in mice and rats, indicating that anaphylactoid reactions may happen in clinical therapy.

关 键 词：穿琥宁注射液 类过敏反应 实验研究 

分 类 号：R285.1[医药卫生—中药学]