SPF级和野生布氏田鼠血液学指标的比较  

Comparison of hematological parameters between specific pathogen-free Brandt′s vole and Brandt′s vole raised in open environment

作　　者：潘思丹 施海霞 宋铭晶 

机构地区：[1]中国医学科学院医学实验动物研究所北京协和医学院比较医学中心卫生部人类疾病比较医学重点实验室国家中医药管理局人类疾病动物模型三级实验室,北京100021

出　　处：《中国卫生检验杂志》2012年第11期2630-2632,共3页Chinese Journal of Health Laboratory Technology

基　　金：国家科技支撑计划课题(2009BAI83B01);北京市自然科学基金资助项目(7122110)资助

摘　　要：目的:了解不同微生物控制程度(屏障环境和普通环境)对布氏田鼠血液学指标的影响。比较SPF级布氏田鼠(specific pathogen-free,SPF)和野生布氏田鼠血液学指标的差异。方法:选取4月龄SPF级布氏田鼠和野生布氏田鼠各16只,雌雄各半,测定12项血液学指标。结果:实验室开放环境饲养的野生布氏田鼠血红蛋白指标低于SPF级布氏田鼠,红细胞分布宽度和血小板数高于SPF级布氏田鼠。部分血液学指标在不同性别之间差异较大。结论:普通开放条件下饲养的野外捕获的布氏田鼠与SPF级布氏田鼠的部分血液学指标存在显著差异,说明微生物控制能影响动物的血液指标。Objective：To investigate the effect of different microbial environment（conventional and barrier） on hematological parameters of Brandt′s voles.To compare the difference of hematologic parameters between Brandt′s voles from specific pathogen-free closed colony and wild populations breeding in open environment.Methods： 16 individuals（male∶ female=1∶ 1） of 4-month-old Brandt′s vole were chosen from pathogen-free closed colony and wild population respectively.Twelve hematological parameters of the Brandt′s voles were analyzed.Results： Brandt′s voles captured in wild population and raised in open environment had lower value of HGB,higher value of RDW and PLT than the Brandt′s vole taken from specific pathogen-free closed colony and raised in the barrier.Conclusion： There are significant differences of hematologic parameters between specific pathogen-free Brandt′s vole and Brandt′s vole raised in open environment,indicating that the microbial control can affect the animal′s blood indicators.

关 键 词：布氏田鼠 血液学指标 SPF级 

分 类 号：R332[医药卫生—人体生理学]